The role of autophagy in Parkinson's disease: rotenone-based modeling
- Nian Xiong†1,
- Jing Xiong†1,
- Min Jia†1,
- Ling Liu1,
- Xiaowei Zhang1,
- Zhenzhen Chen1,
- Jinsha Huang1,
- Zhentao Zhang2,
- Lingling Hou1,
- Zhijian Luo3,
- Devina Ghoorah4,
- Zhicheng Lin5, 6 and
- Tao Wang1Email author
© Xiong et al.; licensee BioMed Central Ltd. 2013
Received: 29 August 2012
Accepted: 6 March 2013
Published: 15 March 2013
Autophagy-mediated self-digestion of cytoplasmic inclusions may be protective against neurodegenerative diseases such as Parkinson’s disease (PD). However, excessive autophagic activation evokes autophagic programmed cell death.
In this study, we aimed at exploring the role of autophagy in the pathogenesis of rotenone-induced cellular and animal models for PD.
Reactive oxygen species over-generation, mitochondrial membrane potential reduction or apoptosis rate elevation occurred in a dose-dependent fashion in rotenone-treated human neuroblastoma cell line SH-SY5Y. The time- and dose-dependent increases in autophagic marker microtubule-associated protein1 light chain 3 (LC3) expression and decreases in autophagic adaptor protein P62 were observed in this cellular model. LC3-positive autophagic vacuoles were colocalized with alpha-synuclein-overexpressed aggregations. Moreover, the number of autophagic vacuoles was increased in rotenone-based PD models in vitro and in vivo.
These data, along with our previous finding showing rotenone-induced toxicity was prevented by the autophagy enhancers and was aggravated by the autophagy inhibitors in SH-SY5Y, suggest that autophagy contributes to the pathogenesis of PD, attenuates the rotenone toxicity and possibly represents a new subcellular target for treating PD.
KeywordsAutophagy LC3 Parkinson's Desease Rotenone Autophagosome Pathogenesis
Parkinson’s Disease (PD) is the second most common neurodegenerative disease and affects as many as 1–2 % of the worldwide population aging at 60 years and older . The pathological hallmarks of PD include the loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNc) and DA terminals in the striatum, and the presence of proteinaceous cytoplasmic inclusions called Lewy bodies. Currently, the precise pathogenic mechanisms in PD remain incompletely understood. As Lewy Bodies contain aggregated alpha-synuclein, ubiquitin and other misfolded proteins , a growing attention has been drawn to the role of autophagy in the pathogenesis of PD . Different from ubiquitin-proteasome system which degrades proteins through the narrow barrel of proteasome, macroautophagy (referred to below as autophagy) is responsible for the largely non-specific bulk degradation of long-lived cytosolic proteins and organelles.
It is acknowledged that autophagy is related to PD. Autophagosomes containing neuromelanin and lipofuscin have been identified in degenerating neurons in brains with PD . Moreover, overexpressions of alpha-synuclein mutants have been reported to activate autophagy [4–6]. Rapamycin (Rap), known as an autophagy inducer, is neuroprotective in parkinsonian cellular and mice models, by enhancing autophagy to degrade misfolded proteins . Our previous findings suggested that valproic acid and carbamazepine (the most commonly used anti-epilepsy and mood-stabilizing medications with low-risk and easy administration), as well as Rap and lithium, might be potential therapeutics for PD as autophagy enhancers . On the other hand, 3-methyladenine, an autophagy inhibitor, has reportedly offered neuroprotection against 6-hydroxydopamine toxicity , suggesting that excessive activation of autophagy during neuronal loss participates in the pathogenesis pathway of PD . That is to say, both autophagy inducer and inhibitor have been proven to serve as neuroprotectors against PD. There are still many controversial and unsolved problems regarding the role of autophagy in PD. First, whether it is autophagy activation or autophagy suppression that confers neuroprotection against PD; second, whether autophagy is a defense mechanism or a response to the DA neuron death; third, whether autophagy is a key mechanism or just an innocent bystander in the pathogenesis of PD [11, 12]. Therefore, we need to better understand the role of autophagy in the pathogenesis of PD prior to any clinical application of autophagy-based medications in PD subjects.
Rotenone, a potent mitochondrial complex I inhibitor, is one of the most relevant neurotoxins to induce parkinsonian symptoms [6, 13–15]. Despite debates, the rotenone model is able to recapitulate slow and specific loss of DA neurons and over-expression of alpha-synuclein and better mimics the clinical features of idiopathic PD [16–20]. Among the various models for PD, the rotenone model has recently drawn particular attention for two reasons: 1) it reproduces most of the motor symptoms and the histopathological features of PD, including Lewy bodies [21, 22]; and 2) rotenone and other pesticides are powerful inhibitors of mitochondrial respiration and associated with the higher incidence of sporadic Parkinsonism among the population of rural areas [23–26]. Thus, rotenone-induced parkinsonian models were chosen to explore the role of autophagy in PD in this study. We found that rotenone induced time- and dose-dependent apoptosis of SH-SY5Y cells increases the autophagic marker microtubule-associated protein1 light chain 3 expression, and increases the number of autophagic vacuoles, and decreases the autophagic adaptor protein P62 expression. These data indicated that autophagy was involved in the pathogenesis of rotenone-induced PD models, revealing a neuroprotective alternative to treating PD.
SH-SY5Y cells (American type culture collection, gift from Dr. Jianguo Chen) were cultured in DMEM/F12 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (Invitrogen) at 37°C with 5% CO2 and 95% air (vol/vol). Rotenone (Sigma-Aldrich, St. louis, MO, USA) was dissolved in dimethyl sulfoxide before dilution with the culture medium. The final concentration of dimethyl sulfoxide (DMSO) per well was 0.2%. DMSO alone was added to the culture medium in control group (“Con-group”). For the dose-dependent study, rotenone was given at a concentration of 0.1, 0.5, 1, 2.5, 5, 10 and 20 μM for 24 hours. For the time-dependent study, rotenone (2.5μM) was given for 3, 6, 12, 24, 36 or 48 hours to induce cell damage.
Cell viability was assessed by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method [27, 28]. The MTT assay is a colorimetric assay of the activity of cellular enzymes that reduce the tetrazolium dye, MTT, into insoluble formazan, giving a purple color. Briefly, SH-SY5Y cells were plated at a density of 1 × 104 cells per well in 96-well plates. After exposure to rotenone and vehicle, 20 μl of MTT (5 mg/ml, Sigma-Aldrich) was added into each well before incubation in a humidified incubator at 37°C for 4 hours to allow the formation of purple formazan crystal. Then, 100 μl of the solubilization reagent (0.1 N HCl in anhydrous isopropanol, Sigma-Aldrich) was added into each well and lysate spectrophotometrically measured for absorption at λ 570 nm with background subtraction at 690 nm. Cell viability was expressed as a percentage of the value in untreated control cells.
Detection of apoptosis, mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) in SH-SY5Y cells
Annexin V was used to probe phosphatidylserine expression on the cell surface, an event found in apoptosis as well as other forms of cell death . In this study, staurosporine (50nM, Sigma-Aldrich) treatment for 24 hours was employed as a positive control to induce cell apoptosis. SH-SY5Y cells were harvested after treatment with 0.25% trypsin, washed with phosphate buffered solution (PBS) and incubated in PBS containing the Annexin V-fluorescein isothiocyanate (Annexin V, 5 μl in 100 μl PBS) and Propidium Iodide (PI, 100 μg/ml working solution, 1 μl in 100 μl) at 37°C in darkness for 15 minutes. The apoptosis rate = [Annexin V(+)PI(−) cells + Annexin V(+)PI(+) cells] /total cell × 100%. The specific fluorescence of 10,000 cells was analyzed on FACScalibur (BD Biosciences, Franklin Lakes, NJ, USA) within 1 hour after antigen antibody reaction [27–29]. Data were analyzed by using FSC express version 3.0 (De Novo Software, Los Angeles, CA, USA).
It was reported that a decrease in MMP was one of the earliest events in apoptosis . When stained with JC-1, red fluorescence of mitochondria was due to the formation of J-aggregates at high MMP, and green fluorescence of mitochondria to the formation of JC-1 monomers at low MMP . ROS detection was based on ROS-catalyzed formation of fluorescent compound DCF. The nonfluorescent probe DCFH-DA could diffuse passively through the cellular membrane. With intracellular esterase activity, DCFH-DA formed a nonfluorescent compound DCFH, which was oxidized into the fluorescent compound DCF by ROS . For the analysis of MMP and ROS, cells were harvested, resuspended in PBS and immediately stained with JC-1 (1 mg/ml in DMSO, Molecular Probes, Eugene, OR, USA)  or DCFH-DA (10 μM, Invitrogen) , and incubated at 37°C for 30 minutes in the darkness. After washing with ice-cold PBS twice, the samples were subject to FACScan flow Cytometry. Data were analyzed again by using FSC express version 3.0 (De Novo Software).
The microtubule-associated protein1 light chain 3 (LC3) was a marker for all types of autophagic vacuolar organelles. A higher LC3 expression level meant more autophagic vacuolar organelles in the cells. It was reported that LC3 expression level could be related to the induction of autophagy or a block of autophagy and subsequent accumulation of LC3 [33–35]. The mammalian proteins p62 and NBR1 were selectively degraded by autophagy and could act as cargo receptors or adaptors for the autophagic degradation of ubiquitinated substrates [36, 37]. The conversion of LC3-I into LC3-II (LC3-II level compared to LC3-I level) and the expression of P62 (which was degraded by autophagy) were indicative of autophagic activity. Higher ratio of LC3-II/LC3-I and lower p62 expression means higher autophagic activity. In this study, LC3 and p62 levels were measured by an immunoblotting method [27, 28, 38]. Cells were rinsed twice with cold PBS and lysed in buffer (50 mM Tris–HCl, pH 7.5, 100 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS,1 mM EDTA, 1 mM sodium orthovanadate,10 mM sodium fluoride, and 100 mg/ml PMSF). After incubation on ice for 30 minutes, cell lysates were then clarified by centrifugation at 12,000 × g and 4°C for 10 minutes and the supernatant saved for protein analysis and Western blotting. Total protein concentration was determined by the BCA kit (Sigma-Aldrich). Equal amounts of proteins (30 μg) were fractionated by 15% SDS-PAGE, and transferred to nitrocellulose membrane. The membrane was blocked with 5% non-fat milk in Tris-buffered saline (TBS) for 1 hour at room temperature, followed by incubation with primary antibodies against LC3, P62 (Sigma-Aldrich, St. Louis, MO, USA) and β-actin (Santa Cruz, Santa Cruz, CA, USA) overnight at 4°C. The membranes were then washed twice with TBS tween-20 and probed with the corresponding secondary antibodies conjugated with HRP at room temperature for 1 hour. Detection was carried out using an enhanced chemiluminescence detection kit (Pierce, Rockford, IL, USA), followed by autoradiography. The relative intensity of bands was quantified using Quantity One analysis system (Quantity One, Hercules, CA, USA). All data from three independent experiments were expressed as the ratio to optical density values of the corresponding controls for statistical analyses.
SH-SY5Y cells grown on cover slips were fixed with 4% paraformaldehyde at 4°C for 30 minutes, washed with PBS and permeabilized with 0.1% Triton-X100 and 5% bovine serum albumin (Invitrogen) in PBS , followed by incubation at 4°C overnight with the LC3 antibody (1:100, rabbit polyclonal antibody, Sigma-Aldrich, St. Louis, MO, USA) without or with alpha-synuclein (SNCA) antibody (1:100, mouse monoclonal antibody, Billerica, MA, USA). The corresponding secondary FITC-conjugated donkey-anti-rabbit IgG (1:200, vol/vol, Proteintech, Chicago, IL, USA) without or with Cy3-conjugated goat-anti-mouse IgG (1:500, vol/vol, Proteintech, Chicago, IL, USA) diluted in 10 μg/ml Hoechst 33258 (Sigma-Aldrich) was applied at room temperature for 1 hour. Cells were observed by using a confocal microscope (Olympas, Tokyo, Japan) and the images (50 per group, repeat for 3 times) were analyzed by a design-based unbiased method and a morphometry/image analysis system (Image-Pro Plus 6.0 software package, Bethesda, MD, USA; Edit-Convert to-Gray Scale- Enhance-Invert contrast-Apply contrast –Measure-Count/Size-Measure-Density Mean).
Rotenone-induced hemiparkinsonian rat model
This study was approved by the Ethical Committee on Animal Experimentation of Tongji Medical College, Huazhong University of Science and Technology, China. The rotenone-induced stereotaxical hemiparkinsonian rat (inbred adult female Sprague–Dawley rats, 220–260 g) model was used in this study . Briefly, animals were anesthetized with chloral hydrate (400 mg/kg in 0.9% NaCl, i.p.) and fastened on a cotton bed over a stereotaxic frame (RWD Life Science, Shenzhen, China). Rotenone dissolved in DMSO (3 μg/μl) was infused into the right ventral tegmental area (AP: 5.0 mm; L: 1.0 mm; DV: 7.8 mm) at a flow rate of 0.2 μl/minute. The needle was left in place for additional 5 minutes for complete diffusion of the drug. Rotenone was infused into the right SNc (AP: 5.0 mm; L: 2.0 mm; DV: 8.0 mm) at a flow rate of 0.2 μl/minute, with a 5-minute needle retention. After needle withdrawal, proper postoperative care was given until the animals recovered completely. The animals were administrated with ibuprofen and penicillin in the drinking water for 24 hours to alleviate potential postsurgical discomfort and to prevent infection.
The preparation for electron microscopy (EM) was described previously . Harvested by detaching with 0.25% trypsin, SH-SY5Y were washed twice in PBS, and then fixed in 0.01 M PBS containing 2.5% glutaraldehyde. For the brain tissues, the animals were sacrificed 1 day, 2 days, 1 week, 2 weeks or 4 weeks after the stereological surgery. A 1-mm3 tissue block from the left and right SNc regions (−4.5 to −6.2 mm caudal to the bregma) was micro-punched, fixed in PBS containing 2.5% glutaraldehyde, and preserved at 4°C for further processing. The fragments were post-fixed in 1% osmium tetroxide in the same buffer, dehydrated in graded alcohols, embedded in Epon 812, sectioned with an ultramicrotome, and stained with uranyl acetate and lead citrate. The sections were examined with a transmission electron microscope (TEM; Technai 10, Philips, the Netherlands). For the SH-SY5Y cell-based study, the ultrastructural images were quantified by randomly counting of 100 cells and assessing the percentage of the cells with one or more autophagic vacuoles . For the animals study, three blocks of SNc from each group were sectioned discontinuously for 5 slices, and 50 cell profiles (with a nucleus) were examined on each grid for counting the autophagic vacuoles per cell profile at X 13,500 magnification [40, 41]. Each experiment was conducted in triplicate.
Statistical analysis was carried out by using SPSS version 12.0 for Windows software (SPSS, Chicago, IL, USA). Given a normal distribution in all groups, intergroup differences were assessed by one-way analysis of variance (ANOVA) followed by Least square difference's post hoc test . Results are presented as mean ± SEM, with P value of < 0.05 as statistically significant.
Rotenone affected SH-SY5Y cell proliferation time- and dose-dependently
Rotenone induced dose- and time-dependent apoptosis, MMP reduction and ROS generation in cultured SH-SY5Y cells
Rotenone up-regulated LC3 expression and down-regulated P62 expression in SH-SY5Y at an early stage after administration
Data from the time-dependence study indicated the ratio of LC3-II to LC3- I in Rot-12h, Rot-24h, Rot-36h and Rot-48h group was significant higher than that in Con-group. There was no significant difference between Rot-3h, Rot-6h and Con-groups (Figure 3B, E). The expression of P62 in Rot-3h, Rot-12h, Rot-24h, Rot-36h and Rot-48h group was 23.48%, 58.82%, 90.02%, 68.32% and 93.02% lower than control group (Figure 3B, F). These data indicated the activation of autophagy pathway in SH-SY5Y cells at the early stage after rotenone infusion (within 24 hours after rotenone treatment).
Rotenone induced autophagic vacuole formation in SH-SY5Y cells and SNc neurons
Rotenone-induced ultrastructural changes in SNc (semi-quantification)
Mitochondrial crest fracture
Mitochondrial vacuolar degeneration
Dilated and broken rough endoplasmic reticula
Perinular space augmentation
increase of autophagic vacuoles/lysosomes
Degeneration of medullary sheathes
Contralateral SNc (Figure 6A)
Mitochondrial complex ribosomes, medullary sheathes
One day after surgery (Figure 6B)
Two day after surgery (6C)
One week after surgery (Figure6E)
Two weeks after surgery (6F)
Four weeks after surgery (Figure 6G,H)
In this study, we have shown that 1) rotenone induces dose- and time-dependent apoptosis, MMP reduction and ROS generation in SH-SY5Y; 2) rotenone causes time- or dose-dependent upregulation of LC3 expression and decrease in P62 expression in SH-SY5Y; 3) rotenone induces autophagic vacuole formation in SH-SY5Y cells and SNc neurons and 4) LC3-positive autophagic vacuoles are colocalized with SNCA-overexpressed aggregations. These data demonstrate the involvement of autophagy in rotenone-induced parkinsonian models both in vitro and in vivo.
We suggest that autophagy activation offers neuroprotection against rotenone-caused parkinsonian. In this study, the data showed a time-dependent activation of autophagy at the first 36 hours after rotenone administration and a dramatic decrease in autophagy level in these cells 48 hours after rotenone treatment. Autophagy is an important cellular response to stress like toxins and oxidative stress. The accumulation of autophagic vacuoles in the cytoplasm of SH-SY5Y cells may be attributable to rotenone-induced toxicity via oxidative stress and mitochondrial dysfunction. These findings are similar to the results from previous studies which showing that oxidative stress may be upstream process of autophagy . The activation of autophagy may help to prevent cell damage as a compensatory auto-regulative mechanism. However, when the overload of pathogenic stress exceeds cellular compensation capability, the autophagy may be under the ability to maintain the cellular balance and ultimately lead to cell death . The neuroprotective effects of Rap and the neurotoxic effects of Chl on these models further confirm that the autophagy enhancement is protective .
Previous studies have shown that both autophagy inhibition and enhancement are neuroprotective [7, 9, 46, 47]. The difference may be attributed to different models, different mechanisms involved in these models and different treatment phases. Increasing evidence demonstrates that early-stage activation of autophagy is protective and late-stage over activation of autophagy eventually leads to cell death. Late-stage neuronal cell loss generally occurs via autophagy . Abnormal manipulation of autophagy can result in autophagic cell death or protein-aggregated neurodegeneration [49–52]. Therefore, precise autophagy regulation rather than massive autophagy enhancement or inhibition should be a therapeutic direction of PD.
We suggest that autophagy is a key mechanism involved in DA cell death rather than an innocent bystander for following reasons. 1) An increase in autophagy-related structures has been found in parkinsonian patients  and models, suggesting autophagy is involved; 2) pretreatment of SH-SY5Y cells with the autophagy enhancer Rap is neuroprotective while pretreatment of SH-SY5Y cells with the autophagy inhibitor Chl is toxic ; and 3) genetically selective manipulation of autophagy-related genes causes neurodegeneration and behavioral deficits in animals [49–51, 54].
Autophagy is involved in the pathogenesis of rotenone-induced PD; autophagy enhancement provides a potential therapeutic alternative for PD. Moreover, autophagy is a defense mechanism responsive to rotenone stress for the DA cell death. Enhancement of autophagy confers neuroprotection against rotenone toxicity. However, which autophagy enhancer, such as Rap, lithium, valproic acid, carbamazepine or trehalose, would be the most suitable one for PD patients remains unknown. Further endeavors are needed to address how to maintain proper cellular autophagy level, as well as the safety issues regarding long-term application of autophagy-related drugs to PD subjects.
Microtubule-associated protein1 light chain 3
Substantia nigra pars compacta
Phosphate buffered solution
Mitochondrial membrane potential
Reactive oxygen species
- Annexin V:
Annexin V-fluorescein isothiocyanate
Tris buffered saline
Transmission electron microscope.
This work was supported by grants 30870866, 81071021 and 31171211 from the National Natural Science Foundation of China (to TW), grant 81200983 from the National Natural Science Foundation of China (to NX), grant 81100958 from the National Natural Science Foundation of China (to ZTZ), grant 20066002100 from the Wuhan Science and Technology Bureau, China (to TW) and grant 2012B09 from the China Medical Foundation (to NX).
- Olanow CW, Stern MB, Sethi K: The scientific and clinical basis for the treatment of Parkinson disease (2009). Neurology. 2009, 72 (21 Suppl 4): S1-136.View ArticlePubMed
- Verhoef LG, Lindsten K, Masucci MG, Dantuma NP: Aggregate formation inhibits proteasomal degradation of polyglutamine proteins. Hum Mol Genet. 2002, 11 (22): 2689-2700. 10.1093/hmg/11.22.2689.View ArticlePubMed
- Xilouri M, Vogiatzi T, Vekrellis K, Stefanis L: alpha-synuclein degradation by autophagic pathways: a potential key to Parkinson's disease pathogenesis. Autophagy. 2008, 4 (7): 917-919.View ArticlePubMed
- Cuervo AM, Stefanis L, Fredenburg R, Lansbury PT, Sulzer D: Impaired degradation of mutant alpha-synuclein by chaperone-mediated autophagy. Science. 2004, 305 (5688): 1292-1295. 10.1126/science.1101738.View ArticlePubMed
- Vogiatzi T, Xilouri M, Vekrellis K, Stefanis L: Wild type alpha-synuclein is degraded by chaperone-mediated autophagy and macroautophagy in neuronal cells. J Biol Chem. 2008, 283 (35): 23542-23556. 10.1074/jbc.M801992200.PubMed CentralView ArticlePubMed
- Xiong N, Huang J, Zhang Z, Xiong J, Liu X, Jia M, Wang F, Chen C, Cao X, Liang Z: Stereotaxical infusion of rotenone: a reliable rodent model for Parkinson's disease. PLoS One. 2009, 4 (11): e7878-10.1371/journal.pone.0007878.PubMed CentralView ArticlePubMed
- Pan T, Kondo S, Zhu W, Xie W, Jankovic J, Le W: Neuroprotection of rapamycin in lactacystin-induced neurodegeneration via autophagy enhancement. Neurobiol Dis. 2008, 32 (1): 16-25. 10.1016/j.nbd.2008.06.003.View ArticlePubMed
- Xiong N, Jia M, Chen C, Xiong J, Zhang Z, Huang J, Hou L, Yang H, Cao X, Liang Z: Potential autophagy enhancers attenuate rotenone-induced toxicity in SH-SY5Y. Neuroscience. 2011, 199: 292-302.View ArticlePubMed
- Li L, Wang X, Fei X, Xia L, Qin Z, Liang Z: Parkinson's disease involves autophagy and abnormal distribution of cathepsin L. Neurosci Lett. 2011, 489 (1): 62-67. 10.1016/j.neulet.2010.11.068.View ArticlePubMed
- Bredesen DE, Rao RV, Mehlen P: Cell death in the nervous system. Nature. 2006, 443 (7113): 796-802. 10.1038/nature05293.PubMed CentralView ArticlePubMed
- Cheung ZH, Ip NY: The emerging role of autophagy in Parkinson's disease. Mol Brain. 2009, 2 (1): 29-10.1186/1756-6606-2-29.PubMed CentralView ArticlePubMed
- Rami A: Review: autophagy in neurodegeneration: firefighter and/or incendiarist?. Neuropathol Appl Neurobiol. 2009, 35 (5): 449-461. 10.1111/j.1365-2990.2009.01034.x.View ArticlePubMed
- Betarbet R, Sherer TB, MacKenzie G, Garcia-Osuna M, Panov AV, Greenamyre JT: Chronic systemic pesticide exposure reproduces features of Parkinson's disease. Nat Neurosci. 2000, 3 (12): 1301-1306. 10.1038/81834.View ArticlePubMed
- Feng Y, Liang ZH, Wang T, Qiao X, Liu HJ: Sun SG: alpha-Synuclein redistributed and aggregated in rotenone-induced Parkinson's disease rats. Neurosci Bull. 2006, 22 (5): 288-293.PubMed
- Xiong N, Long X, Xiong J, Jia M, Chen C, Huang J, Ghoorah D, Kong X, Lin Z, Wang T: Mitochondrial complex I inhibitor rotenone-induced toxicity and its potential mechanisms in Parkinson's disease models. Crit Rev Toxicol. 2012, 42 (7): 613-632. 10.3109/10408444.2012.680431.View ArticlePubMed
- Xiong N, Zhang Z, Huang J, Chen C, Jia M, Xiong J, Liu X, Wang F, Cao X, Liang Z: VEGF-expressing human umbilical cord mesenchymal stem cells, an improved therapy strategy for Parkinson's disease. Gene Ther. 2011, 18 (4): 394-402. 10.1038/gt.2010.152.View ArticlePubMed
- Xiong N, Cao X, Zhang Z, Huang J, Chen C, Jia M, Xiong J, Liang Z, Sun S, Lin Z: Long-term efficacy and safety of human umbilical cord mesenchymal stromal cells in rotenone-induced hemiparkinsonian rats. Biol Blood Marrow Transplant. 2010, 16 (11): 1519-1529. 10.1016/j.bbmt.2010.06.004.View ArticlePubMed
- Cannon JR, Tapias V, Na HM, Honick AS, Drolet RE, Greenamyre JT: A highly reproducible rotenone model of Parkinson's disease. Neurobiol Dis. 2009, 34 (2): 279-290. 10.1016/j.nbd.2009.01.016.PubMed CentralView ArticlePubMed
- Pan-Montojo F, Anichtchik O, Dening Y, Knels L, Pursche S, Jung R, Jackson S, Gille G, Spillantini MG, Reichmann H: Progression of Parkinson's disease pathology is reproduced by intragastric administration of rotenone in mice. PLoS One. 2010, 5 (1): e8762-10.1371/journal.pone.0008762.PubMed CentralView ArticlePubMed
- Greenamyre JT, Cannon JR, Drolet R, Mastroberardino PG: Lessons from the rotenone model of Parkinson's disease. Trends Pharmacol Sci. 2010, 31 (4): 141-142. 10.1016/j.tips.2009.12.006. author reply 142–143. -PubMed CentralView ArticlePubMed
- Betarbet R, Sherer TB, Greenamyre JT: Animal models of Parkinson's disease. Bioessays. 2002, 24 (4): 308-318. 10.1002/bies.10067.View ArticlePubMed
- Schober A: Classic toxin-induced animal models of Parkinson's disease: 6-OHDA and MPTP. Cell Tissue Res. 2004, 318 (1): 215-224. 10.1007/s00441-004-0938-y.View ArticlePubMed
- Landrigan PJ, Sonawane B, Butler RN, Trasande L, Callan R, Droller D: Early environmental origins of neurodegenerative disease in later life. Environ Health Perspect. 2005, 113 (9): 1230-1233. 10.1289/ehp.7571.PubMed CentralView ArticlePubMed
- Priyadarshi A, Khuder SA, Schaub EA, Priyadarshi SS: Environmental risk factors and Parkinson's disease: a metaanalysis. Environ Res. 2001, 86 (2): 122-127. 10.1006/enrs.2001.4264.View ArticlePubMed
- Dhillon AS, Tarbutton GL, Levin JL, Plotkin GM, Lowry LK, Nalbone JT, Shepherd S: Pesticide/environmental exposures and Parkinson's disease in East Texas. J Agromedicine. 2008, 13 (1): 37-48. 10.1080/10599240801986215.View ArticlePubMed
- Tanner CM, Kamel F, Ross GW, Hoppin JA, Goldman SM, Korell M, Marras C, Bhudhikanok GS, Kasten M, Chade AR: Rotenone, paraquat, and Parkinson's disease. Environ Health Perspect. 2011, 119 (6): 866-872. 10.1289/ehp.1002839.PubMed CentralView ArticlePubMed
- Ma R, Xiong N, Huang C, Tang Q, Hu B, Xiang J, Li G: Erythropoietin protects PC12 cells from beta-amyloid(25–35)-induced apoptosis via PI3K/Akt signaling pathway. Neuropharmacology. 2009, 56 (6–7): 1027-1034.View ArticlePubMed
- Zhang Z, Cao X, Xiong N, Wang H, Huang J, Sun S, Liang Z, Wang T: DNA polymerase-beta is required for 1-methyl-4-phenylpyridinium-induced apoptotic death in neurons. Apoptosis. 2010, 15 (1): 105-115. 10.1007/s10495-009-0425-8.View ArticlePubMed
- Koopman G, Reutelingsperger CP, Kuijten GA, Keehnen RM, Pals ST, van Oers MH: Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood. 1994, 84 (5): 1415-1420.PubMed
- Wadia JS, Chalmers-Redman RM, Ju WJ, Carlile GW, Phillips JL, Fraser AD, Tatton WG: Mitochondrial membrane potential and nuclear changes in apoptosis caused by serum and nerve growth factor withdrawal: time course and modification by (−)-deprenyl. J Neurosci. 1998, 18 (3): 932-947.PubMed
- Salvioli S, Ardizzoni A, Franceschi C, Cossarizza A: JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 1997, 411 (1): 77-82. 10.1016/S0014-5793(97)00669-8.View ArticlePubMed
- Giardino I, Edelstein D, Brownlee M: BCL-2 expression or antioxidants prevent hyperglycemia-induced formation of intracellular advanced glycation endproducts in bovine endothelial cells. J Clin Invest. 1996, 97 (6): 1422-1428. 10.1172/JCI118563.PubMed CentralView ArticlePubMed
- Cherra SJ, Kulich SM, Uechi G, Balasubramani M, Mountzouris J, Day BW, Chu CT: Regulation of the autophagy protein LC3 by phosphorylation. J Cell Biol. 2010, 190 (4): 533-539. 10.1083/jcb.201002108.PubMed CentralView ArticlePubMed
- Kouroku Y, Fujita E, Tanida I, Ueno T, Isoai A, Kumagai H, Ogawa S, Kaufman RJ, Kominami E, Momoi T: ER stress (PERK/eIF2alpha phosphorylation) mediates the polyglutamine-induced LC3 conversion, an essential step for autophagy formation. Cell Death Differ. 2007, 14 (2): 230-239. 10.1038/sj.cdd.4401984.View ArticlePubMed
- Wang AL, Boulton ME, Dunn WA, Rao HV, Cai J, Lukas TJ, Neufeld AH: Using LC3 to monitor autophagy flux in the retinal pigment epithelium. Autophagy. 2009, 5 (8): 1190-1193. 10.4161/auto.5.8.10087.PubMed CentralView ArticlePubMed
- Rusten TE, Stenmark H: p62, an autophagy hero or culprit?. Nat Cell Biol. 2010, 12 (3): 207-209. 10.1038/ncb0310-207.View ArticlePubMed
- Lamark T, Kirkin V, Dikic I, Johansen T: NBR1 and p62 as cargo receptors for selective autophagy of ubiquitinated targets. Cell Cycle. 2009, 8 (13): 1986-1990. 10.4161/cc.8.13.8892.View ArticlePubMed
- Huang J, Hao L, Xiong N, Cao X, Liang Z, Sun S, Wang T: Involvement of glyceraldehyde-3-phosphate dehydrogenase in rotenone-induced cell apoptosis: relevance to protein misfolding and aggregation. Brain Res. 2009, 1279: 1-8.View ArticlePubMed
- Cooney R, Baker J, Brain O, Danis B, Pichulik T, Allan P, Ferguson DJ, Campbell BJ, Jewell D, Simmons A: NOD2 stimulation induces autophagy in dendritic cells influencing bacterial handling and antigen presentation. Nat Med. 2010, 16 (1): 90-97. 10.1038/nm.2069.View ArticlePubMed
- Berg TO, Fengsrud M, Stromhaug PE, Berg T, Seglen PO: Isolation and characterization of rat liver amphisomes. Evidence for fusion of autophagosomes with both early and late endosomes. J Biol Chem. 1998, 273 (34): 21883-21892. 10.1074/jbc.273.34.21883.View ArticlePubMed
- Kaiser CA, Schekman R: Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway. Cell. 1990, 61 (4): 723-733. 10.1016/0092-8674(90)90483-U.View ArticlePubMed
- Wang ZF, Li HL, Li XC, Zhang Q, Tian Q, Wang Q, Xu H, Wang JZ: Effects of endogenous beta-amyloid overproduction on tau phosphorylation in cell culture. J Neurochem. 2006, 98 (4): 1167-1175. 10.1111/j.1471-4159.2006.03956.x.View ArticlePubMed
- Liu C, Gao Y, Barrett J, Hu B: Autophagy and protein aggregation after brain ischemia. J Neurochem. 2010, 115 (1): 68-78. 10.1111/j.1471-4159.2010.06905.x.PubMed CentralView ArticlePubMed
- Gonzalez-Polo RA, Niso-Santano M, Ortiz-Ortiz MA, Gomez-Martin A, Moran JM, Garcia-Rubio L, Francisco-Morcillo J, Zaragoza C, Soler G, Fuentes JM: Inhibition of paraquat-induced autophagy accelerates the apoptotic cell death in neuroblastoma SH-SY5Y cells. Toxicol Sci. 2007, 97 (2): 448-458. 10.1093/toxsci/kfm040.View ArticlePubMed
- Trojanowski JQ, Lee VM: "Fatal attractions" of proteins. A comprehensive hypothetical mechanism underlying Alzheimer's disease and other neurodegenerative disorders. Ann N Y Acad Sci. 2000, 924: 62-67.View ArticlePubMed
- Xue L, Fletcher GC, Tolkovsky AM: Autophagy is activated by apoptotic signalling in sympathetic neurons: an alternative mechanism of death execution. Mol Cell Neurosci. 1999, 14 (3): 180-198. 10.1006/mcne.1999.0780.View ArticlePubMed
- Canu N, Tufi R, Serafino AL, Amadoro G, Ciotti MT, Calissano P: Role of the autophagic-lysosomal system on low potassium-induced apoptosis in cultured cerebellar granule cells. J Neurochem. 2005, 92 (5): 1228-1242. 10.1111/j.1471-4159.2004.02956.x.View ArticlePubMed
- Takacs-Vellai K, Bayci A, Vellai T: Autophagy in neuronal cell loss: a road to death. Bioessays. 2006, 28 (11): 1126-1131. 10.1002/bies.20489.View ArticlePubMed
- Hara T, Nakamura K, Matsui M, Yamamoto A, Nakahara Y, Suzuki-Migishima R, Yokoyama M, Mishima K, Saito I, Okano H: Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice. Nature. 2006, 441 (7095): 885-889. 10.1038/nature04724.View ArticlePubMed
- Komatsu M, Waguri S, Chiba T, Murata S, Iwata J, Tanida I, Ueno T, Koike M, Uchiyama Y, Kominami E: Loss of autophagy in the central nervous system causes neurodegeneration in mice. Nature. 2006, 441 (7095): 880-884. 10.1038/nature04723.View ArticlePubMed
- Komatsu M, Waguri S, Koike M, Sou YS, Ueno T, Hara T, Mizushima N, Iwata J, Ezaki J, Murata S: Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell. 2007, 131 (6): 1149-1163. 10.1016/j.cell.2007.10.035.View ArticlePubMed
- Klionsky DJ: Neurodegeneration: good riddance to bad rubbish. Nature. 2006, 441 (7095): 819-820. 10.1038/441819a.View ArticlePubMed
- Anglade P, Vyas S, Javoy-Agid F, Herrero MT, Michel PP, Marquez J, Mouatt-Prigent A, Ruberg M, Hirsch EC, Agid Y: Apoptosis and autophagy in nigral neurons of patients with Parkinson's disease. Histol Histopathol. 1997, 12 (1): 25-31.PubMed
- Komatsu M, Wang QJ, Holstein GR, Friedrich VL, Iwata J, Kominami E, Chait BT, Tanaka K, Yue Z: Essential role for autophagy protein Atg7 in the maintenance of axonal homeostasis and the prevention of axonal degeneration. Proc Natl Acad Sci U S A. 2007, 104 (36): 14489-14494. 10.1073/pnas.0701311104.PubMed CentralView ArticlePubMed
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