Figure 1
Schematic of experimental design. Rats were exposed to control handling or SPS, followed by 7 or 14 days of quiescent period with no manipulation. Next, for the Control, SPS 7d and SPS 14d groups, subsequent forced swim (FS), open-field (OF) and elevated plus maze (EPM) test were performed, and the rats were finally sacrificed for Western blotting and real-time RT-PCR. For the Controlā+āSaline, Controlā+āZIP, SPSā+āSaline, and SPSā+āZIP groups, ZIP or saline were administrated after the 7 days of quiescent period of SPS. Following another 7 days interval, FS, OF and EPM were performed.