Dopamine immunoreactive fibres in each of the quantified regions. Representative photomicrographs, taken from a saline control, of dopamine (DA) immunoreactive fibres of each of the quantified regions. A.1: Prefrontal cortex; A.2: Layer VI of the prelimbic area; A.3: Layer IV of the lateral orbital and agranular insular areas. B.1: Nucleus accumbens (NAC); B.2: Medial shell of NAC; B.3: Lateral core of NAC; B.4: Olfactory tubercle. C.1: Amygdala (AMY); C.2: Central nucleus of AMY; C.3: Basolateral nucleus of AMY. Note the differential innervation pattern and density of DA fibres in the respective regions. Scale bars: 1000 μm (A.1, B.1, C.1); 50 μm (A.2-3, B.2-4, C.2-3).