Figure 1

The top trace is a typical 215 nm normal elution pattern from a control on c-18 reverse phase column. (Vydac C-18 protein and peptide column, Vydac, Hesperia, Ca 250 × 4.5 mm) at 30 degrees C [11]. First morning urine equivalent to 250 nano-moles of creatinine was applied. The peaks after hippuric acid were integrated by the HPLC attached computer. The bottom trace is from a severely depressed female patient age 33 yr (Diagnosis: F: 33.3).