Figure 6

Effects of DLPhtEtn on hippocampal size and the number of hippocampal neurons. (A) Eight coronal sections were prepared from the brain for SAMR1 and SAMP8 mice after a series of behavioral tests. Hippocampal area (yellow lines in the right column) both on the left and right sides was measured, and areas from 8 sections per a mouse were summated. Bar, 1 mm. (B) Values represent the mean (± SEM) hippocampal size (n = 5 for SAMR1 mice treated with PEG, 10 for SAMP8 mice treated with PEG and 7 for SAMP8 mice treated with DLPhtEtn). (C) For coronal sections from the brain for SAMR1 and SAMP8 mice with oral administration with PEG or DLPhtEtn (1 mg/kg) for 7 months, the number of neurons immunoreactive to an anti-NeuN antibody was counted in the consistent area (142 μm × 192 μm) of the hippocampal CA1 region both on the left and right sides (black dots in the right column) from 8 sections per a mouse and summated. Bar, 50 μM. (D) Values represent the mean (± SEM) number of hippocampal neurons (n = 5 for SAMR1 mice treated with PEG, 10 for SAMP8 mice treated with PEG and 7 for SAMP8 mice treated with DLPhtEtn). P values, unpaired t-test.