Long term effects of siAAH and pAAH intracerebroventricular transfection on Notch signaling and plasticity proteins. P2 Long Evans rat pups were administered intracerebroventricular injections of (A-D; I-L) siAAH or siScr, or (E-H; M-P) recombinant plasmid pAAH or pGFP cDNA. Cerebellar tissue harvested from P21 rats was used to measure immunoreactivity to (A, E) AAH-A85G6 antibody, (B, F) NOTCH-1, (C, G) Jagged-1, (D, H) β-actin, (I.M) GFAP, (J, N) synaptophysin, (K, O) GAP-43, and (L, P) SNAP-25 by direct binding ELISA. Immunoreactivity was detected with horseradish peroxidase conjugated secondary antibody and the Amplex Red fluorophore. Fluorescence was measured in a Spectramax M5 microplate reader (Ex 530 nm/Em 590 nm). Panels display box plots with means and minimum-maximum whiskers. Inter-group statistical comparisons were made using Student T-tests. Significant P-values are indicated within the panels.