Figure 6From: Role of aspartyl-(asparaginyl)-β-hydroxylase mediated notch signaling in cerebellar development and functionLong term effects of siAAH and pAAH intracerebroventricular transfection on Notch signaling and plasticity proteins. P2 Long Evans rat pups were administered intracerebroventricular injections of (A-D; I-L) siAAH or siScr, or (E-H; M-P) recombinant plasmid pAAH or pGFP cDNA. Cerebellar tissue harvested from P21 rats was used to measure immunoreactivity to (A, E) AAH-A85G6 antibody, (B, F) NOTCH-1, (C, G) Jagged-1, (D, H) β-actin, (I.M) GFAP, (J, N) synaptophysin, (K, O) GAP-43, and (L, P) SNAP-25 by direct binding ELISA. Immunoreactivity was detected with horseradish peroxidase conjugated secondary antibody and the Amplex Red fluorophore. Fluorescence was measured in a Spectramax M5 microplate reader (Ex 530 nm/Em 590 nm). Panels display box plots with means and minimum-maximum whiskers. Inter-group statistical comparisons were made using Student T-tests. Significant P-values are indicated within the panels.Back to article page