Figure 10

MHCI-HC is upregulated in primary visual cortex neurons that receive afferents from the intact eye. A: Example of the region analyzed for optical density measurements (delineated with white, dashed line). Abbreviations: primary visual cortex, V1; monocularly enucleated animal, ME. B: Optical density profiles recorded from layer IV of the primary visual cortex of a control animal (black dotted line) and two enucleated animals (red and blue dotted lines). C: Width of MHCI-HC immunoreactive column-like patches showing that the wider columns display stronger MHCI-HC staining indicating that MHCI-HC levels are elevated in neurons receiving afferents from the intact eye. Difference between the groups is highly significant as determined by Student's two tailed t-test: **, p < 0.01 (t = 5.166 df = 4). Data are expressed as mean ± SEM (standard error of the mean), N = 3 animals/group, 12 measurements per animal. Note: Black horizontal lines in (A) and (B) indicate areas with high MHCI-HC immunoreactivity, while white lines point to low immunoreactivity areas.