Table 1 Information of the primers and PCR-RFLP Analysis of seven SNPs in DISC1 gene
SNP | Position | Primer sequence (5'-3') | Product (bp) | RFLP | Allele (bp) | |
|---|---|---|---|---|---|---|
rs4366301 | Intron 1 | F: AGAAGACTAGGAAAAATAACT R: ATAAACACTGAACAGAATGTC | 406 | ScrFI | C: (43/265/98) | G: (43/363) |
rs11585959a | Intron 2 | F: TGACATTCTACCTTCTCTCTC R: ACTACCTTTATTACCATCTTC | 556 | - | - | - |
rs1322784 | Intron 6 | F: CCTCCTCTGTTGAAAGTAGGT R:GGAAGAAAGTCTGAATGTGAC | 645 | Van91I | A: (201/444) | G: (645) |
rs6668845 | Intron 9 | F:AAAAAAAAAAAATCAACTGAG R:CCCAAATCTTTCATAGTGACT | 572 | TaiI | G: (442/130) | A: (572) |
rs10864698 | Intron 9 | F: TCCAGAGCCAGTGAAATGTTC R: TTGTGCCTGAATGAATGAGAC | 630 | MunI | A: (381/249) | G: (630) |
rs872624 | Intron 9 | F: ACAAAACCAGAAACCTTGAGT R: ACATATTAGGGAAACTGAATG | 757 | NdeI | G: (506/251) | A: (757) |
rs821616 | Exon 11 | F: GTATTGGGCTGCTGAGTCTG R: GACCTCTTTCTGTTCACCTCC | 540 | Bsrl | T: (204/336) | A: (540) |