Long-term effects of environmentally relevant doses of 2,2',4,4',5,5' hexachlorobiphenyl (PCB153) on neurobehavioural development, health and spontaneous behaviour in maternally exposed mice

Table 2 Feed concentration, body weight, reproductive output and feed intake by reproducing dams fed PCB153 in a fish or a casein-based diet.

Diet (n)	PCB153 ng/g feed	Initial BW dams	Final BW dams	Repr. succ %	Litter size	Intake gestation (g/day)	Intake lactation (g/day)	Gestation dose (μg/kgBW*day)	Lactation dose (μg/kgBW*day)
Casein Control (0)	0.25 ± 0.12	n.d	n.d	0	n.d	n.d	n.d	n.d	n.d
Fish Control (3)	0.50 ± 0.07	28.9 ± 0.5	30.4 ± 0.2	33	4.7(4-5)	3.6 ± 0.2	7.7 ± 0.4	0.05 ± 0.00	0.12 ± 0.00
Casein Low PCB (3)	6.53 ± 0.12	29.1 ± 0.5	31.1 ± 0.9	33	6.3 (5-9)	3.6 ± 0.1	8.3 ± 0.7	0.73 ± 0.06	1.72 ± 0.23
Fish Low PCB (5)	6.57 ± 0.67	29.6 ± 0.8 (7)	31.3 ± 0.6 (7)	78	7.0 (4-9)	3.6 ± 0.1	8.6 ± 0.5	0.69 ± 0.03	1.63 ± 0.11
Casein High PCB (6)	1400 ± 60	28.4 ± 0.9	31.3 ± 1.5	56	5.8 (4-8)	3.9 ± 0.2	8.1 ± 0.3*	170 ± 10	350 ± 20*
Fish High PCB (5)	1500 ± 300	30.0 ± 1.0	29.6 ± 0.7	56	6.8 (4-10)	3.5 ± 0.2	9.2 ± 0.3*	160 ± 10	420 ± 20*

Data are presented as mean ± SEM. Number of litters per group in brackets. Four litters were born in the Casein Control group, but no pups lived until postnatal day 5. Initial BW = BW after acclimation, final BW = BW end of weaning. Repr. succ: = percent successful reproduction in relation to the number of mated females. Litter size is given as mean (min-max). Feed intake during gestation (GD0-21) and lactation (PND0-21) was based on daily monitoring of feed intake. Daily doses were calculated based on average feed intake in grams and PCB153 concentrations in feeds, rounded off to the nearest ten ng/g for the high levels. Two females with single pups in Fish Low PCB were excluded from the calculation of litter size, feed intake and dose during gestation and lactation, but were included for comparisons of initial and final body weight (BW) of reproducing dams. n.d: No data due to reproductive failure. *: Groups that share a symbol are significantly different (Student's t-test: p < 0.05).

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