DRD2 and PPP1R1B (DARPP-32) polymorphisms independently confer increased risk for autism spectrum disorders and additively predict affected status in male-only affected sib-pair families

Table 1 PCR and digestion conditions for DRD2 and PPP1R1B markers used in this study

*DRD2* Marker (5′ → 3′)¹	Primers	[MgCl²]	Annealing temperature	# of cycles	Restriction enzyme (U)²
rs1799732 Ins/Del	F 5′-GAGAAGACTGGCGAGCAGAC-3′	1.5 mM	63°C	35	BstNI (0.05)
	R 5′-CCACCAAAGGAGCTGTACCT-3′
rs1079597 G/A³	F 5′-GATACCCACTTCAGGAAGTC-3′	1.0 mM	55°C	34	TaqI (0.4)
	R 5′-CAGTAAAGAACTAGGAGTCAG-3′
rs1800498 T/C³	F 5′-CCCAGCAGGGAGAGGGAGTA-3′	1.0 mM	55°C	34	TaqI (0.4)
	R 5′-GACAAGTACTTGGTAAGCATG-3′
rs1800497 C/T³	F 5′-CCGTCGACGGCTGGCCAAGTTGTCTA-3′	1.0 mM	58°C	34	TaqI (0.4)
	R 5′-CCGTCGACCCTTCCTGAGTGTCATCA-3′
*PPP1R1B* Marker (5′ → 3′)	Primers	[MgCl ² ]	Annealing temperature	# of cycles	Restriction enzyme (U) ²
rs1495099 G/C	F 5′-TTGTTGCTGAGCTGAGATGC-3′	1.0 mM	60°C	35	PvuII (0.3)
	R 5′-CTCCAGGGAAATGCACAAAG-3′
rs907094 T/C	F 5′-ACCTGATTGGGAGAGGGACT-3′	1.0 mM	60°C	34	MseI (0.3)
	R 5′-GTAAGCTGAGGGGCCTGTG-3′
rs3764352 A/G	F 5′-CTGTTTTGGAGGGGTCTCAG-3′	1.0 mM	60°C	35	BccI (0.3)
	R 5′-TGGGAATACTGAAGAGTCAACC-3′

¹Rs1799732 Ins/Del, rs1079597 G/A, rs1800498 T/C, and rs1800497 C/T previously known as −141 C Ins/Del, TaqI B, TaqI D, and TaqI A, respectively.
²Restriction enzymes obtained from New England Biolabs, Pickering, ON, Canada
³Primer sequences have been reported previously[43].

ISSN: 1744-9081