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Table 1 PCR and digestion conditions for DRD2 and PPP1R1B markers used in this study

From: DRD2 and PPP1R1B (DARPP-32) polymorphisms independently confer increased risk for autism spectrum disorders and additively predict affected status in male-only affected sib-pair families

DRD2 Marker (5′ → 3′)1 Primers [MgCl2] Annealing temperature # of cycles Restriction enzyme (U)2
rs1799732 Ins/Del F 5′-GAGAAGACTGGCGAGCAGAC-3′ 1.5 mM 63°C 35 BstNI (0.05)
  R 5′-CCACCAAAGGAGCTGTACCT-3′     
rs1079597 G/A3 F 5′-GATACCCACTTCAGGAAGTC-3′ 1.0 mM 55°C 34 TaqI (0.4)
  R 5′-CAGTAAAGAACTAGGAGTCAG-3′     
rs1800498 T/C3 F 5′-CCCAGCAGGGAGAGGGAGTA-3′ 1.0 mM 55°C 34 TaqI (0.4)
  R 5′-GACAAGTACTTGGTAAGCATG-3′     
rs1800497 C/T3 F 5′-CCGTCGACGGCTGGCCAAGTTGTCTA-3′ 1.0 mM 58°C 34 TaqI (0.4)
  R 5′-CCGTCGACCCTTCCTGAGTGTCATCA-3′     
PPP1R1B Marker (5′ → 3′) Primers [MgCl 2 ] Annealing temperature # of cycles Restriction enzyme (U) 2
rs1495099 G/C F 5′-TTGTTGCTGAGCTGAGATGC-3′ 1.0 mM 60°C 35 PvuII (0.3)
  R 5′-CTCCAGGGAAATGCACAAAG-3′     
rs907094 T/C F 5′-ACCTGATTGGGAGAGGGACT-3′ 1.0 mM 60°C 34 MseI (0.3)
  R 5′-GTAAGCTGAGGGGCCTGTG-3′     
rs3764352 A/G F 5′-CTGTTTTGGAGGGGTCTCAG-3′ 1.0 mM 60°C 35 BccI (0.3)
  R 5′-TGGGAATACTGAAGAGTCAACC-3′     
  1. 1Rs1799732 Ins/Del, rs1079597 G/A, rs1800498 T/C, and rs1800497 C/T previously known as −141 C Ins/Del, TaqI B, TaqI D, and TaqI A, respectively.
  2. 2Restriction enzymes obtained from New England Biolabs, Pickering, ON, Canada
  3. 3Primer sequences have been reported previously[43].