Skip to main content
Figure 2 | Behavioral and Brain Functions

Figure 2

From: The role of autophagy in Parkinson's disease: rotenone-based modeling

Figure 2

Rotenone evoked apoptosis, MMP reduction and ROS generation dose- and time-dependently in SH-SY5Y. For Annexin V/PI double-staining, the apoptosis rate was a sum of both early and late apoptosis rates (apoptosis rate = [Annexin V(+)PI(−) cells + Annexin V(+)PI(+) cells] /total cell × 100%), staurosporine (STS, 50nM, Sigma-Aldrich) treatment for 24 hours was used as a positive control to induce cell apoptosis. The apoptosis rate of Con-, Rot-0.1μM, Rot-0.5μM, Rot-1μM, Rot-2.5μM, Rot-5μM, Rot-10μM, Rot-20μM and STS-group was 2.60±0.90%, 3.00±0.60%, 4.30±1.20%, 4.80±1.80%, 6.50±0.98%, 7.20±2.10%, 13.00±3.00%, 19.00±2.70% and 42.31±7.90%, respectively. The JC-1 staining was used to assess the MMP of SH-SY5Y cells, the percentages of JC-1 monomers presented the percentages of SH-SY5Y cells with low MMP. The relative intensity of DCF was employed to evaluate the ROS level in SH-SY5Y cells. (A), (D): Statistical analysis of apoptosis rates in different groups. (B), (E): Relative percentage of JC-1 monomers-positive cells (cells with low MMP) in different groups. (C), (F): Fluorescence intensity of DCFH in all groups. (*P < 0.05, compared to Con-group).

Back to article page