Figure 4

LC3 immunostaining in rotenone-treated SH-SY5Y. Immunostaining was performed to assess the LC3/SNCA expression and distribution in SH-SY5Y cells treated with or without rotenone (2.5μM for 24 hours). The nuclei were stained by Hoechst33258. (A, B) LC3 immunofluorescence staining for Con- and Rot-2.5μM group; (C, D) LC3/SNCA double immunostaining for Con- and Rot-2.5μM group; (E) statistical analysis of relative mean fluorescence intensity of LC3 immunofluorescence staining (Scale bar = 20 μm; *P < 0.05, compared to Con-group).