Effect of environmental manipulation in neuropathic and sham control mice. Nerve injury-induced hypersensitivity to mechanical (A) and cold (B) stimuli was attenuated following environmental enrichment and exacerbated by environmental impoverishment (mechanical only). (C) The motor impairment observed following nerve injury was insensitive to environmental change. (D) Environmental enrichment had significant antidepressant effects in uninjured but not in neuropathic mice. Impoverishment had no effect on this assay in either group. ** p < 0.01, *** p < 0.001, 2-way repeated measures ANOVA followed by the Bonferronni post-hoc test, enriched vs. impoverished within pathological condition (neuropathic vs. sham); # p < 0.05, ## p < 0.05, 1-way ANOVA followed by the Dunnett post-hoc test, effect of time within pathological condition; mean ± SEM, n = 8-9/group.