Figure 3

Principal component analysis. The PCA analysis was based on the gene expression patterns in male (M) and female (F) zebrafish brain regions with and without E2 exposure. Gene expression in the forebrain, midbrain and hindbrain regions were analyzed. a Score plot showing the distribution of the brain regions in control and E2 exposed zebrafish based on gene expression. The ellipse shows Hotellings T² (0.05). b PCA loading plot showing the distribution of the measured gene expression levels.