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Fig. 1 | Behavioral and Brain Functions

Fig. 1

From: Thrombin-induced microglial activation impairs hippocampal neurogenesis and spatial memory ability in mice

Fig. 1

CD68 staining of activated microglia/microphage cells in the SVZ. a The schematic diagram of the animal use and the timeline of experiments. The training or adaptive time for the rotarod and Morris water maze group was 2 days, and for beam walking was 3 days. The mice were tested at day 1, 2, and 3 for water maze, at day 5 for the probe trial, and at day 1, 3, 5 for rotarod and beam walking tests. The animals were sacrificed at day 5. be The representative CD68 staining and DAPI nucleus staining near thrombin (1U) injection site in sham (b), ICH (c), ICH + hirudin (d), and ICH + indo (e) groups. f The CD68 staining at the injection tract. The right lateral ventricle (LV) and the right SVZ were located in the vicinity of the thrombin infusion tract. SGZ of hippocampus was distal to the injection tract and could not be seen on the graph. g The amplified square area in c. h The CD68 abundance was normalized to sham group. After the treatment of thrombin (1U), the CD68 signal of activated microglia cells in the SVZ area increased significantly in comparison to sham group, indicating an increase in the number and/or hypertrophy of microglia cells. The administration of hirudin at 20 min after the injection of thrombion (1U) significantly inhibited the increase of CD68 abundance. The administration of indo also significantly decreased the CD68 abundance. **p < 0.01, compared with sham; ##p < 0.01, compared with ICH group. LV lateral ventricle. Blue DAPI; red CD68; “” transplantation tract, n = 10 mice/group; scale bar 50 µm

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