Fig. 3

Cell proliferation measured as DCX⁺/BrdU⁺ staining in the dentate gyrus of hippocampus. a Sham mice. b 1U thrombin injured mice. The brain injury caused by thrombin decreased the DCX⁺/Brdu⁺ cells in the mouse dentate gyrus of dorsal hippocampus. c Mice received intrastriatal injection of thrombin and treated with indo. d Mice received intrastriatal injection of thrombin and treated with hirudin (a thrombin inhibitor). e Total number of BrdU⁺/DCX⁺ cells counted in the DG of all groups. Compared with the sham group, BrdU⁺/DCX⁺ cell number was significantly decreased by 1U thrombin injection, and was reversed by hirudin administration. f 1U thrombin injection significantly decreased the DCX⁺ cells in SGZ region as compared with sham. Hirudin and indomethacin treatment after 1U thrombin injection significantly ameliorated the DCX positive cell losses. **p < 0.01, compared with sham; ^#p < 0.05, compared with ICH group. Blue DAPI; green BrdU; red DCX; n = 10 mice/group; scale bar 50 µm