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Fig. 3 | Behavioral and Brain Functions

Fig. 3

From: Acute immobilization stress following contextual fear conditioning reduces fear memory: timing is essential

Fig. 3

Epigenetic modification of BDNF promoter via acute immobilization stress applied after fear training (Experiments 3 and 4). a Experimental protocol for contextual fear conditioning training followed by immobilization stress. b Representative western blots for the acetylation of H3K14 in the hippocampus at 90 and 120 min after the training and quantification of immunoblot densities for mean (± SEM) acetylated H3K14 at 90 and 120 min after the training compared with that in the without training group (n = 4–5). c Acetylated H3K14 levels in the hippocampus at bdnf promoter 3 at 90 min after the training compared with that in the no training group (n = 3–4). d Levels of H3K14 acetylation in the hippocampus at bdnf promoter 3 at 90 min after the training compared with that in the group without training (n = 3–4). e Levels of H3K14 acetylation in the hippocampus at bdnf promoter 4 at 90 min after the training compared with that in the group without training (n = 4). Data are expressed as mean ± SEM. *P < 0.05 compared with training 90 min. # P < 0.05 compared with training 120 min. Different sets of mice were used for western blotting and ChIP assay analyses

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