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Fig. 1 | Behavioral and Brain Functions

Fig. 1

From: Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) improves brain ischemia-induced pulmonary injury in rats associated to TNF-α expression

Fig. 1

Experiment design for transplantation of BMSCs in rat with brain ischemia-induced pulmonary injury. mNSS modified neurological severity score for measuring neural function after brain ischemia. TTC staining 2, 3, 5-Triphenyltetrazolium chloride for measuring the viability of brain tissue. ICC immunocytochemistry staining to identify the BMSCs. qRT-PCR quantitative real-time polymerase chain reaction for detecting the mRNA expression of TNF-α. HE staining hematoxylin-eosin staining. W/D ratio wet-to-dry weight ratio of lung. WB western blot analysis for quantifying the protein level of TNF-α. IHS immunohistochemistry staining for analyzing the expression of TNFα. ISH in situ hybridization for determining the location of TNF-α in lung with cerebral ischemia. BI brain ischemia. BMSCs brain ischemia with BMSCs transplantation

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