Fig. 2
Cerebral ischemia induced by MCAO produces lung injury in rat. a, b Morphological examination of the cerebral cortex neurons by H&E staining. a Sham group; b BI group. a In Sham group, there were abundant pyramidal cells. b After brain ischemia, the nuclei of pyramidal cells (bold arrow) were shrunken and surrounded with acidophilic cytoplasm. c mNSS score in the Sham group and BI group at 24 h after MCAO. d, e H&E staining of the lung tissue in Sham group (d) and BI group (e). Red blood cells (bold arrow) were observed in the pulmonary alveoli (e). f Bar graph of the numbers of red cells in the lung in Sham group and BI group was shown. g Wet-to-dry weight (W/D) ratio of lung in Sham group and BI group was shown. h Viability of brain tissue was shown by TTC staining in rats suffered MCAO. i Bar graph of the infarct volume percentage in Sham group and BI group were shown. *P < 0.05;**P < 0.01, n = 5 in each group. Scale bars a,b, d, e, 25 μm; h, 200 μm