Fig. 4

Representative histopathological finding of TNF-α in the injured lung after brain ischemia. a, b Immunohistochemistry staining of TNF-α in type I (brown signal, bold arrow), II lung epithelial cells (brown signal, thin arrow) and macrophagocytes (brown signal, arrow head) in the Sham group (a) and BI group (b). c, d ISH of TNF-α (red signals) was detected in the typeI (ISH, red signals, bold arrow), II lung epithelial cells (ISH, red signals, thin arrow) and macrophagocytes (ISH, red signals, arrow head) in the Sham group (c) and BI group (d). Scale bars a–d, 25 μm