Skip to main content

Table 2 Primer sequences and conditions for real-time PCR and the following HRM analysis of the examined MDR1 C3435T

From: Association between C3435T polymorphism of MDR1 gene and the incidence of drug-resistant epilepsy in the population of Polish children

Target

Primers (5′–3′)

PCR cycling (40 cycles)

HRM

Denaturation

Annealing

Extension

rs1045642

Forward: TCCTGAAGTTGATCTGTGAAC

Reverse: AGTGACTCGATGAAGGCA

30 s for 95 °C

30 s for 58 °C

30 s for 72 °C

75–90 °C

Back to article page

Behavioral and Brain Functions

ISSN: 1744-9081

Contact us