Skip to main content
Fig. 2 | Behavioral and Brain Functions

Fig. 2

From: Pristane induced lupus mice as a model for neuropsychiatric lupus (NPSLE)

Fig. 2

Examination for lupus-like symptoms in PIL mice. A Representative images showing lipogranulomas (arrow) adherent to the abdominal surface of the diaphragm in PIL mice. B Serum levels of cytokines (IL-1β, IFN-α, IFN-β, IFN-γ, IL-10, IL-6, IL-17A, TNF-α, TWEAK, BAFF and APRIL), chemokines (CCL2, CXCL10 and CCL7), autoantibodies (anti-chromatin, anti-dsDNA, anti-nRNP and anti-Sm) and total IgG, as detected by ELISA. IL-1β (group effect: F1,22 = 144.5, p < 0.0001; time effect: F3.991,87.81 = 5.593, p = 0.0005; interaction effect: F5,110 = 6.255, p < 0.0001); IFN-α (p < 0.0001, 0.0472, 0.0754); IFN-β (p < 0.0001, < 0.0001, < 0.0001); IFN-γ (p < 0.0001, < 0.0001, < 0.0001); IL-10 (p < 0.0001, < 0.0001, < 0.0001); IL-6 (p < 0.0001, < 0.0001, < 0.0001); IL-17A (p < 0.0001, < 0.0001, < 0.0001); TNF-α (p < 0.0001, 0.3646, 0.3835); TWEAK (p = 0.0657, 0.7505, 0.9879); BAFF (p = 0.0538, 0.1978, 0.6215); APRIL (p = 0.2328, 0.8839, 0.8463); CCL2 (p < 0.0001, 0.0004, < 0.0001); CXCL10 (p < 0.0001, < 0.0001, 0.0037); CCL7 (p = 0.0985, 0.1661, 0.7891); anti-chromatin (p < 0.0001, 0.0004, < 0.0001); anti-dsDNA (p < 0.0001, < 0.0001, < 0.0001); anti-nRNP (p < 0.0001, < 0.0001, < 0.0001); anti-Sm (p < 0.0001, 0.0091, 0.0012) and total IgG (p < 0.0001, < 0.0001, < 0.0001). C Left panel showing representative images of glomeruli stained with H&E. Right panel showing representative images of frozen kidney sections stained for IgG and C3. D Quantitative analysis of the MFI of IgG (p < 0.0001, < 0.0001, < 0.0001) and C3 (p < 0.0001, < 0.0001, < 0.0001) in glomeruli. E Quantitative analysis of Scr (p < 0.0001, < 0.0001, < 0.0001), BUN (p < 0.0001, 0.0430, 0.0024) and 24 h proteinuria (p < 0.0001, 0.0001, < 0.0001). F Upper panel showing representative images of the hind paw, and quantitative analysis of arthritis severity score (p < 0.0001, 0.0004, < 0.0001). Lower panel showing representative images of joint tissues with H&E staining, and quantitative analysis of synovial inflammatory score (p < 0.0001, < 0.0001, < 0.0001). G Representative images of spleen, and quantitative analysis of spleen index (p = 0.0007, 0.0013, 0.0001). The data are expressed as the mean ± SEM (n = 12 in each group). Two-way ANOVA followed by Tukey’s post hoc test or Scheirer–Ray–Hare test: *p < 0.05, **p < 0.01

Back to article page