Fig. 8

Examination for lipofuscin deposition in the cortex and hippocampus. A and C Representative images of the cortex and hippocampus stained with H&E. B and D Representative images showing autofluorescent lipofuscin at 480 nm (green) and 550 nm (red) exciting light in the cortex and hippocampus. DAPI staining for nuclei (blue). White square showing the co-localization of an enlarged neuron (blue) with autofluorescence (green and red). E and F Quantification analysis of lipofuscin foci in the cortex (p < 0.0001, < 0.0001, < 0.0001) and hippocampus (p < 0.0001, < 0.0001, < 0.0001). The data are expressed as the mean ± SEM (n = 12 in each group). Two-way ANOVA followed by Tukey’s post hoc test or Scheirer–Ray–Hare test: **p < 0.01