Fig. 3

GFAP + astrocytes in the CA3 hipocampal subregion are visualized in (A). Effects of intervention (pseudo vs. WBV) and genotype (J20 vs. WT) on expression of GFAP positive cells in the CA1, CA3, DG and Hilus regions are depicted in B. Significantly decreased coverage of GFAP positive cells in the J20 animals was detected in the CA1 and Hilus regions (B, CA1 and Hilus). The same trend was observed in the CA3 and Hilus subregions (B, CA3 and Hilus). Significant effect of interaction (intervention vs. genotype) was detected in the CA1 and CA3 subregions (B, CA1 and CA3). Additional posthoc analysis revealed that WT—WBV and pseudo WBV groups showed significantly higher coverage compared to the J20 – pseudo WBV group in the CA1 region (B, CA1). Significantly increased GFAP coverage was detected in the J20—WBV and WT—pseudo WBV groups compared to the J20—pseudo WBV group in the CA3 region (B, CA3). In addition, significant increase of GFAP coverage was detected by WBV in the Hilus region (Panel B, Hilus). Representative images of GFAP + astrocytes were taken about the CA3 hippocampal subregion at 200 × magnifications (A). Data are depicted as mean ± SEM. * indicates: P = .05. ** P < .01. *** P < .001. Scale bars in A are 100 um