Whole-body vibration ameliorates glial pathological changes in the hippocampus of hAPP transgenic mice, but does not affect plaque load

Table 1 Effects of intervention (pseudo WBV vs. WBV) and genotype (J20 vs. WT) on microglia number (n), microglia coverage (in %), cell body size and dendrites size measured in pixels, and the number of cells in the CA1, CA3, DG and Hilus subregions of the hippocampus

Group	Region	Microglia number (n)	Total coverage (in %)	Cell body area (px)	Dendrites area (px)
Pseudo WBV—WT	CA1	33 ± 1.9	16 ± 0.9	237 ± 15	5647 ± 342
	CA3	33 ± 2.0	16 ± 1.1	244 ± 12	6094 ± 531
	DG	31 ± 1.9	16 ± 0.9	270 ± 16	4993 ± 393
	Hilus	25 ± 2.0	15 ± 1.1	262 ± 21	4201 ± 344
Pseudo WBV—J20	CA1	33 ± 1.1	13 ± 0.6 +	353 ± 39 +	5728 ± 332
	CA3	32 ± 2.0	13 ± 0.5 +	328 ± 42 +	5734 ± 510
	DG	33 ± 1.0	12 ± 0.4 +	365 ± 41 +	4978 ± 277
	Hilus	21 ± 1.5	11 ± 0.6 +	398 ± 41 +	3464 ± 214
WBV—WT	CA1	36 ± 2.7	16 ± 0.8	257 ± 17	5952 ± 404*
	CA3	32 ± 1.8	16 ± 0.7	252 ± 14	5948 ± 379
	DG	31 ± 1.9	16 ± 0.7	267 ± 14	6129 ± 261*
	Hilus	21 ± 2.0	14 ± 0.8	271 ± 12	4460 ± 308
WBV—J20	CA1	34 ± 1.9	15 ± 0.7 +	348 ± 45 +	6950 ± 163*
	CA3	31 ± 1.6	15 ± 0.9 +	349 ± 35 +	6764 ± 355
	DG	34 ± 1.6	14 ± 0.7 +	368 ± 43 +	6335 ± 299*
	Hilus	22 ± 2.7	11 ± 0.7 +	361 ± 42 +	3829 ± 448

Microglia number was not significantly altered by genotype or intervention. Significantly higher coverage was detected in the J20 mice compared to the WT mice in all subregions. In contrast, cell body size was significantly increased in the J20 animals compared to the WT animals. WBV treatment significantly increased the size of dendritic processes in both the J20 and WT mice in the CA1 and DG areas. Data are depicted as mean ± SEM. + indicates a significant difference between the main factors “wild type vs. J20”
^*indicates a significant difference between main factors “WBV vs. pseudo WBV”. Additional figures related to these parameters can be found in the Additional file 1

ISSN: 1744-9081