Figure 3

Gel filtration on P 2 gels 1.6 × 90 cm in 0.5 M acetic acid of 24 h complete urine of three patients suffering from depression. (ICD F33.3). The patients were on usual food supply but hospitalized. Whatman 3 MM paper filters was used to filter urine. Elution was monitored by measuring 5% aliquots by off line alkaline hydrolysis and ninhydrin colour developed as described [9], and with an equimolar absorption coefficient for peptides measured at 570 nm for each amino acid.