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Table 1 Primer Pairs Used for Quantitative RT-PCR Analysis

From: Role of aspartyl-(asparaginyl)-β-hydroxylase mediated notch signaling in cerebellar development and function

Primers* Direction Sequence (5'→3') Position (mRNA) Amplicon Size (bp)
Hu For CAC TGT GTG AGG GTC CAT CTT CTG 271 50
Hu Rev TCA AGC CAT TCC ACT CCA TCT G 320  
MAG-1 For AAC CTT CTG TAT CAG TGC TCC TCG 18 63
MAG-1 Rev CAG TCA ACC AAG TCT CTT CCG TG 80  
GFAP For TGG TAA AGA CGG TGG AGA TGC G 1245 200
GFAP Rev GGC ACT AAA ACA GAA GCA AGG GG 1444  
Tau For CGC CAG GAG TTT GAC ACA ATG 244 65
Tau Rev CCT TCT TGG TCT TGG AGC ATA GTG 308  
AAH For TGC CTG CTC GTC TTG TTT GTC 666 118
AAH Rev ATC CGT TCT GTA ACC CGT TGG 783  
HES-1 For AGC GCT ACC GAT CAC AAA GT 70 143
HES-1 Rev TCA GCT GGC ATT TTC CTT TT 212  
JAGGED-1 For CTG AGG ACT ACG AGG GCA AG 2041 191
JAGGED-1 Rev ACA GGT GAA TTT GCC TCC TG 2231  
NOTCH-1 For GGT GGA CAT TGA CGA GTG TG 1918 204
NOTCH-1 Rev CCC TTG AGG CAT AAG CAG AG 2121  
18S rRNA For GGA CAC GGA CAG GAT TGA CA 1278 50
18S rRNA Rev ACC CAC GGA ATC GAG AAA GA 1327  
  1. Forward (For) and reverse (Rev) primer pairs used to measure gene expression by qRT-PCR. Positions of the most 5' nucleotides and the amplicon length (base pair, bp) are listed.