Role of aspartyl-(asparaginyl)-β-hydroxylase mediated notch signaling in cerebellar development and function

Table 1 Primer Pairs Used for Quantitative RT-PCR Analysis

Primers*	Direction	Sequence (5'→3')	Position (mRNA)	Amplicon Size (bp)
Hu	For	CAC TGT GTG AGG GTC CAT CTT CTG	271	50
Hu	Rev	TCA AGC CAT TCC ACT CCA TCT G	320
MAG-1	For	AAC CTT CTG TAT CAG TGC TCC TCG	18	63
MAG-1	Rev	CAG TCA ACC AAG TCT CTT CCG TG	80
GFAP	For	TGG TAA AGA CGG TGG AGA TGC G	1245	200
GFAP	Rev	GGC ACT AAA ACA GAA GCA AGG GG	1444
Tau	For	CGC CAG GAG TTT GAC ACA ATG	244	65
Tau	Rev	CCT TCT TGG TCT TGG AGC ATA GTG	308
AAH	For	TGC CTG CTC GTC TTG TTT GTC	666	118
AAH	Rev	ATC CGT TCT GTA ACC CGT TGG	783
HES-1	For	AGC GCT ACC GAT CAC AAA GT	70	143
HES-1	Rev	TCA GCT GGC ATT TTC CTT TT	212
JAGGED-1	For	CTG AGG ACT ACG AGG GCA AG	2041	191
JAGGED-1	Rev	ACA GGT GAA TTT GCC TCC TG	2231
NOTCH-1	For	GGT GGA CAT TGA CGA GTG TG	1918	204
NOTCH-1	Rev	CCC TTG AGG CAT AAG CAG AG	2121
18S rRNA	For	GGA CAC GGA CAG GAT TGA CA	1278	50
18S rRNA	Rev	ACC CAC GGA ATC GAG AAA GA	1327

Forward (For) and reverse (Rev) primer pairs used to measure gene expression by qRT-PCR. Positions of the most 5' nucleotides and the amplicon length (base pair, bp) are listed.

ISSN: 1744-9081