Habituation measure based on response failure after repeated gentle anterior touch. (A) 20 gentle anterior touches each separated by 10 second inter-stimulus interval were given and the response for each touch was scored as 1 if thw worm responded by moving away, and 0 if there was no response or worm continued moving in same direction. The probability of the worms responding to the touch decreased with increasing number of stimuli. (B) The raw data used in Figure-1A was plotted as a bar graph to illustrate that the average habituation point of dop-2(vs105) and gpa-14(pk347) deletion mutants was significantly lower than WT animals (p < 0.05, two tailed student’s t-test, n = 30 for each strain). Error bars represent SEM values.