Fig. 5

Isolation and identification of BMSCs. a, b, c Cellular morphology of BMSCs at 0 h (a) 3-day (b) and the 3rd passage (c) in primary culture which were observed under inverted phase contrast microscope CD29, CD44 and CD45 immunostaining of the 3rd passage of BMSCs were performed. Immunostaining of CD29 (d, red), CD44 (g, red) and CD45 (j) with nucleus stained DAPI (e, h, k respectively, blue) and the merge graphs (f, i, l) were showed. Scale bars a, b, c, 100 μm; d–l, 25 μm