Fig. 2

Microglia visualized by IBA1 staining in the Hilus subregions is depicted in (A). Effects of intervention (pseudo vs. WBV) and genotype (WT vs. J20) on microglia activation in the CA1, CA3, DG and Hilus subregions are depicted in (B). Significant increase of microglia activation was observed in the J20 animals compared to the WT controls in all subregions (B, CA1, CA3, Dentate Gyrus and Hilus). Microglia activation was only significantly decreased by WBV in the DG (B, Dentate Gyrus). Furthermore, WBV treatment significantly increased the size of microglia dendritic processes in the CA1 and DG subregions (C, CA1 and Dentate Gyrus). Expression of CD68 coverage in the CA1 subregion is visualized in D. Significant decrease of CD68 expression in the CA1 area was detected in the J20 animals compared to the WT controls (E, CA1). In addition, a tendency (p = 0.06) of interaction effect (intervention vs. genotype) on CD68 expression was also observed in the CA1 subregion; additional post-hoc analysis showed a significant decrease for the J20—WBV group compared to the WT—WBV group, as well as the same tendency (p = 0.07) compared to the WT—pseudo WBV group (E, CA1). A significant effect of interaction (intervention vs. genotype) in CD68 expression was observed in the DG area; additional post hoc revealed a strong tendency (p = 0.08) of decrease CD68 expression in the J20—WBV treated animals compared to the J20—pseudo WBV controls (E, Dentate Gyrus). CD68 expression was not significantly altered in the CA3 and Hilus areas (E, CA3 and Hilus). Representative Images of IBA1 and CD68 were taken about the Hilus and CA1 subregions at 200 × magnifications to visualze microglia (A and C). Relevant statistical differences are marked in B, C and D. Data are depicted as mean ± SEM. * indicates: P = .05. Scale bars in A and D are 100 um