Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey
- Adema Ribic1, 2, 6Email author,
- Gabriele Flügge1, 5,
- Christina Schlumbohm1,
- Kerstin Mätz-Rensing3,
- Lutz Walter†2 and
- Eberhard Fuchs†1, 4, 5
© Ribic et al; licensee BioMed Central Ltd. 2011
Received: 14 October 2010
Accepted: 4 January 2011
Published: 4 January 2011
Several recent studies have highlighted the important role of immunity-related molecules in synaptic plasticity processes in the developing and adult mammalian brains. It has been suggested that neuronal MHCI (major histocompatibility complex class I) genes play a role in the refinement and pruning of synapses in the developing visual system. As a fast evolutionary rate may generate distinct properties of molecules in different mammalian species, we studied the expression of MHCI molecules in a nonhuman primate, the common marmoset monkey (Callithrix jacchus).
Methods and results
Analysis of expression levels of MHCI molecules in the developing visual cortex of the common marmoset monkeys revealed a distinct spatio-temporal pattern. High levels of expression were detected very early in postnatal development, at a stage when synaptogenesis takes place and ocular dominance columns are formed. To determine whether the expression of MHCI molecules is regulated by retinal activity, animals were subjected to monocular enucleation. Levels of MHCI heavy chain subunit transcripts in the visual cortex were found to be elevated in response to monocular enucleation. Furthermore, MHCI heavy chain immunoreactivity revealed a banded pattern in layer IV of the visual cortex in enucleated animals, which was not observed in control animals. This pattern of immunoreactivity indicated that higher expression levels were associated with retinal activity coming from the intact eye.
These data demonstrate that, in the nonhuman primate brain, expression of MHCI molecules is regulated by neuronal activity. Moreover, this study extends previous findings by suggesting a role for neuronal MHCI molecules during synaptogenesis in the visual cortex.
The major histocompatibility complex (MHC) is a dense cluster of genes found in all jawed vertebrates that encodes a great number of proteins involved in immune responses [1, 2]. A group of these genes, MHC class I, codes for transmembrane glycoproteins responsible for presentation of antigenic peptides to cytotoxic CD8+ T lymphocytes . MHC class I (MHCI) proteins are typically heterotrimers composed of a polymorphic transmembrane heavy chain (HC), a noncovalently attached ß-2-microglobulin subunit, and a short peptide comprising 8-15 amino acids that is derived from self or foreign antigens [4, 5]. In this form, MHCI modulates immune response by interacting in trans with a large number of immune receptors . Additionally, MHCI heavy chains can be detected under certain conditions on the cell surface without ß-2-microglobulin and peptide . It is thought that in this "free heavy chain" form, MHCI molecules may interact in cis with certain receptors and thereby regulate their trafficking [6–8]. Virtually all nucleated cells express MHCI proteins, usually in their heterotrimeric form; however, their expression on neurons was always debated . Despite the controversy, neuronal expression of MHCI under certain conditions has been reported [10, 11] and recent findings on involvement of neuronal MHCI in brain development and synaptic plasticity were of great surprise [12–15]. These and other studies begun to unravel the previously unknown and complicated mechanisms of interactions between the central nervous and the immune system that may have great clinical implications .
Although MHCI genes are well conserved among mammals, there are a number of differences in the organization, structure, and function of these genes between rodents and primates, including humans . The first study on the function of MHCI molecules in the central nervous system (CNS) proposed a role for them in the removal of excess synapses in the developing visual system . The visual system displays two forms of plasticity: visual input-driven, activity-dependent plasticity and activity-independent plasticity . The development of the main structures of the visual system, the thalamus and the primary visual cortex (V1), are at least at certain developmental stages dependent on the activity coming from the retina [19, 20]. The thalamic dorsolateral geniculate nucleus (LGN) is the first relay structure of visual input and is organized into segregated, eye-specific neuronal layers that form upon stimulation by early spontaneous activity of retinal ganglion cells . Neurons of the LGN send their projections to V1 where their activity is needed in proper development of eye-specific populations of neurons assembled in the ocular dominance columns (ODCs; ). Blockade of activity of one eye during development of the visual circuits while leaving the other one intact (monocular deprivation) perturbs the segregation of the LGN neurons into eye-specific layers [19, 21]. In V1, as a consequence of visual deprivation, the population of neurons responsive to the intact eye increases [18, 22]. Monocular deprivation by means of tetrodotoxin-induced block of retinal activity downregulates MHCI expression levels in the LGN . Furthermore, it was reported that MHCI molecules are indispensable for developmental refinement in the LGN, i.e. for removal of excess synapses during normal development [12, 23]. Asides from differences in the structure and function of MHCI genes, primates and rodents differ significantly in terms of structure of their visual systems . To gain an insight into the role of MHCI molecules in the nonhuman primate brain, we investigated the spatiotemporal pattern of expression of MHCI genes in the primary visual cortex of the common marmoset monkey. Our data confirmed that MHCI molecules are expressed in the developing visual cortex of the marmoset monkey and that their levels are regulated by neuronal activity. However, the temporal pattern of their expression during development of the V1 and their expression levels upon monocular enucleation indicated that MHCI molecules investigated here may not be involved in synapse elimination, but rather in synaptogenesis. These results not only validate the potentially important role of neuronal MHCI molecules in visual cortex development, but also point to interesting interspecies differences in their distribution, and potentially their function.
Thirty-six common male marmoset monkeys (Callithrix jacchus) were investigated. The animals were obtained from the breeding colony at the German Primate Center (Göttingen, Germany). All investigations and the experiments were performed to the highest ethical standards according to the relevant local, national and international regulations concerning the use of animals. All research projects were carried out only with authorization from the relevant ethics committees. We employed the minimum number of animals required to obtain consistent data and to avoid animal suffering. For the termination of non-human primates, the legal requirements, guidelines and recommendations set by the national authorities were followed strictly. The use of animals including non-human primates in research in Germany is based on the "Tierschutzgesetz" (Animal Protection Law) of the 25th of May, 1998 (BGBl.I Nr.30 v.29.5.1998, S. 1105) and the EU guidelines 86/609/EEG. The proposals for research conducted in this study are approved by the local Government and a specially appointed Ethical Review Committee (Niedersächsisches Landesamt für Verbraucherschutz und Lebensmittelsicherheit, permit numbers 33.11.425-04-003/08 and 33.11.425-04-026/07).
For expression studies, animals of the following ages were used [25, 26]: postnatal days 1 and 7, and postnatal months 1, 3, 5, 7, 12, and 21 (N = 3 per age). For monocular enucleation (ME), the left eyes of one month-old marmoset monkeys (N = 6; approximate body weight 75 g) were surgically enucleated under general anesthesia. As anesthesia, the animals received 0.1 ml of a premix containing 4 mg/ml alphaxalon and 1.33 mg/ml alphadolon (Saffan®; Schering-Plough Animal Health, Welwyn Garden City, UK), 0.37 mg/ml diazepam, and 0.015 mg/ml glycopyrroniumbromid (Robinul®; Riemser, Germany). Enucleation was carried out as described for pet animals  and the eyehole was filled with Gelastypt® sponge (Sanofi-Aventis, Frankfurt, Germany) as soon as arterial bleeding was no longer visible. The wound was closed with an intracutane suture of vicryl 6-0 (V302H; Ethicon, Norderstedt, Germany). Directly after surgery (day 0) and at days 3 and 5, all animals received an antibiotic (amoxicillin-trihydrate; Duphamox LA®, Fort Dodge, Würselen, Germany). ME animals and six age-matched controls were sacrificed at five months of age .
For in situ hybridization, brains were immediately removed from terminally anesthetized animals [overdose of ketamine (50 mg/ml), xylazine (10 mg/ml), and atropine (0.1 mg/ml)] and the whole visual cortices were quickly dissected (area determined according to [29, 30]), embedded in Tissue Tek (Sakura Finetek, Heppenheim, Germany), flash frozen in liquid nitrogen, and stored at -80°C. Frozen brains were sectioned using a cryostat (Leica CM3050, Bensheim, Germany) and coronal sections (10 μm) of whole visual cortices were thaw-mounted on adhesive silane-coated slides (Histobond, Marienfeld, Laboratory Glassware, Lauda-Königshofen, Germany). For immunohistochemistry, marmosets that were terminally anesthetized (see above), were perfused transcardially with 0.9% saline, followed by 200 ml of fixative containing 4% paraformaldehyde in 0.1 M sodium-phosphate buffer (pH 7.2) for 15 min. The heads were postfixed in the same fixative, and brains were carefully removed from the skulls on the following day. After cryoprotection in 0.1 M phosphate-buffered saline (PBS; 0.1 mM phosphate buffer, 0.9% NaCl, pH 7.2) containing 30% sucrose, serial coronal sections of the entire visual cortices (thickness: 40 μm for expression studies and 60 μm for ME animals and controls) were obtained using a cryostat.
PCR cloning of MHCI transcripts
The isolation of the common marmoset MHC class I heavy chain cDNA sequences was carried out using reverse transcriptase polymerase chain reaction (RT-PCR). One microgram of total brain RNA was reverse transcribed using the oligo (dT) primer and 400 U of reverse transcriptase (Promega, Mannheim, Germany). An aliquot of this reaction was used as a template in a PCR containing primers designed with the Primer3 software . Primers were devised to amplify full-length marmoset MHC class I heavy chain transcripts (accession number: U59637). Primer sequences also included BamHI restriction sites and were as follows: forward 5'-CACGGATCCCACTTTACAAGCCGTGAGAGAC-3', reverse 5'-CACGGATCCCTCCTGTTGCTCTCGGGGGCCTTG-3'. Caja-G*1 (accession number: U59637) was obtained and cloned into the pDrive vector (Qiagen).
In situ hybridization
Cryosections (10 μm) of visual cortices were dried at RT for 20 min, fixed in 4% buffered paraformaldehyde (PFA, pH 7.2), rinsed in PBS, acetylated (0.1 M triethanolamine, 0.25% acetic acid anhydride), washed in PBS, and dehydrated through a graded ethanol series. Caja-G plasmid DNA was linearized and riboprobes were synthesized using T7 and SP6 RNA polymerases (Promega, Madison, WI, USA) for the antisense and sense probe, respectively, in the presence of 9.25 MBq of 33P-UTP (Hartmann Analytic GmbH, Braunschweig, Germany; specific activity, 3,000 Ci/mmol) for 1 h at 37°C. Probes were purified using Microspin S-400 HR columns (Amersham Pharmacia, Freiburg, Germany) and hybridization buffer (50% deionized formamide, 10% dextran sulphate, 0.3 M NaCl, 1 mM EDTA, 10 mM Tris-HCl, pH 8.0, 500 μg/ml tRNA, 0.1 M dithiothreitol, and 1 × Denhardt's solution) was added to yield a final probe activity of 5 × 104 cpm. The hybridization mixture containing the probe was denatured at 70°C for 10 min, cooled to 55°C, and pipetted directly onto sections (80 μl/section). Hybridization was performed for 18 h at 68°C. Sections were subsequently washed in 4 × SSC (0.6 M NaCl, 0.06 M citric acid), 2 × SSC, and 0.5 × SSC for 10 min each at 37°C. After incubation (1 h at 75°C) in 0.2 × SSC, sections were washed twice in 0.1 × SSC, once at 37°C and again at RT, for 10 min each. Finally, sections were dehydrated through graded alcohols, air dried, and exposed to Bio-Max MR film (Amersham Pharmacia) for three days at 4°C. Films were developed and fixed with GBX (Kodak, Rochester, NJ, USA).
Quantitative in situ hybridization
After in situ hybridization, sections were coated with photoemulsion (Kodak NBT2, Rochester, NJ, USA) at 42°C, dried for 90 min at RT, and stored for seven weeks at 4°C in a lightproof container. Exposed slides were developed at 15°C for 5 min (Kodak developer D-19), rinsed twice briefly in H2O, and fixed for 5 min at RT (fixer, Kodak Polymax). Sections were counterstained either with 0.05% toluidine blue in 0.1% disodium tetraborate (Sigma) for expression studies or with methyl green (Sigma) for monocular enucleation studies, cleared in xylol, and coverslipped using mounting medium (Eukitt, Kindler, Freiburg, Germany). Hybridized sections were visualized with a 40× objective (NA = 1.4; Zeiss) under a light microscope (Axioscope, Zeiss) and silver grain quantification was performed on a cell-by-cell basis using ImageJ (U.S. National Institutes of Health, Bethesda, Maryland, USA). Images were obtained from layer IV neurons and, for each area within the primary visual cortex (demarcated according to [29, 30]) two images were acquired, i.e., one using a green filter to eliminate background staining from methyl green and one using white light to later precisely localize neuronal nuclei. A circular counting mask of 15 μm in diameter was used to delineate the region of interest and was placed over neuronal nuclei during counting. Relative optical density (ROD) threshold intensities were optimized to detect exposed silver grains exclusively. The silver grain density within the region of interest was measured. Grain density was compared between layer IV neurons in primary visual cortices of six animals (three slides per animal, minimum 300 neurons per animal were counted) using Student's t test (GraphPad Prism version 4 for Windows, GraphPad Software, San Diego, CA, USA).
To isolate RNA for RT-PCR, brains were immediately removed from terminally anesthetized animals (see above) and whole occipital lobes containing visual cortices were quickly dissected. Total RNA was isolated from both hemispheres of dissected tissue samples from animals at the age of five months (N = 3 controls and N = 3 unilaterally enucleated animals) using the QIAGEN RNeasy kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. As the cortices had to be separated during RNA isolation due to their size, both left and right hemispheres of all animals were treated as independent samples. The integrity and quantity of purified RNA was assessed by spectrophotometry. Complementary DNA (cDNA) was synthesized from mRNA transcripts using oligo (dT)12-18 primers and Superscript II reverse transcriptase (Invitrogen, Karlsruhe, Germany), according to the manufacturer's instructions. The Primer3 software v2.0  was used to design gene-specific primers, with amplicons ranging from 50 to 150 bp in length. The primers used for the detection of MHC class I heavy chain transcripts were: forward 5'-GTGATGTGGAGGAAGAACAGC-3', reverse 5'-CACTTTACAAGCCGTGAGAGA-3' (CajaG*01, accession number U59637). Primers for the detection of GFAP were: forward 5'-AAACGAGTCCCTGGAGAG-3', reverse 5'-TCCTGGTACTCCTGCAAGT-3' (marmoset GFAP, Ensembl transcript number ENSCJAT00000024380). Primers for the detection of c-Fos were: forward 5'-CGAAGGGAAAGGAATAAGAT-3', reverse 5'-GCAGACTTCTCATCTTCCAG-3' (marmoset c-Fos, Ensembl transcript number ENSCJAT00000040535). Primers for the detection of β-actin were: forward 5'-CATCCGCAAAGACCTGTATG-3', reverse 5'-GGAGCAATGACCTTGATCTTC-3' (marmoset ß-actin, accession number DD279463). A quantitative analysis of gene expression was performed using the 7500 Real-time PCR apparatus (Applied Biosystems, Darmstadt, Germany) in combination with Quantitect SYBR green technology (Qiagen). The light cycler was programmed to the following conditions: an initial PCR activation step of 10 min at 95°C, followed by 40 cycling steps (denaturation for 15 s at 95°C, annealing for 30 s at 55°C, and elongation for 60 s at 72°C). Details of the quantitative real-time PCR were described previously . Dissociation curves were generated for all PCR products to confirm that SYBR green emission was detected from a single PCR product . All products were run on 2.5% agarose gels to confirm a single product (Additional file 1). The Caja-G PCR product was additionally sequenced using the BigDye Terminator Sequencing Kit (Applied Biosystems) and forward primer in order to confirm its identity (Additional File 2). The relative abundance of the MHCI-HC, c-Fos and GFAP mRNA transcripts was calculated relative to the mRNA levels of the internal reference gene β-actin and was compared between both left and right cortices of control and enucleated animals using Student's t-test (GraphPad Prism version 4 for Windows).
Immunocytochemistry for light microscopy
Coronal cryosections (40 μm for expression studies and 60 μm for ME animals) of occipital lobes were collected and washed briefly in PBS before the epitope retrieval step. Epitope retrieval was performed by incubating the sections for 20 min in 10 mM sodium citrate buffer preheated to 80°C. Sections were later brought to RT, washed in PBS, and quenched of endogenous peroxidase activity using 30 min incubation at RT in 0.5% H2O2 in distilled water. Sections were then washed in PBS, blocked for 1 h at RT (3% normal horse serum in PBS), incubated for 16 h at 4°C with mouse monoclonal TP25.99 or Q1/28 IgG [34, 35] kindly provided by S. Ferrone, University of Pittsburgh, USA), 1:300 dilution in 3% normal horse serum in PBS; monoclonal mouse W6/32 , 1:300 dilution in 3% normal horse serum in PBS; monoclonal rabbit anti c-Fos (Cell Signalling Technologies, Beverly, MA, USA); monoclonal anti-GFAP (Sigma) 1:200 dilution in 0.01% Triton-X 100 and 3% normal horse serum in PBS; or control mouse IgG (Sigma), and washed again. For c-Fos, W6/32 and GFAP staining, the epitope retrieval step was not performed. Sections were then incubated with biotinylated horse anti-mouse IgG or donkey anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA), 1:200 dilution in 3% normal horse serum in PBS, for 1 h at RT. After washing, sections were incubated with avidin-biotin horseradish peroxidase (Vectastain Elite ABC Kit, Vector Laboratories, Burlingame, CA, USA), 1:100 dilution in 3% normal horse serum in PBS, for 1 h at RT, washed in PBS and then again in 0.05 M Tris/HCl (pH 7.2) prior to DAB detection (DAB detection with or without nickel enhancement was performed according to the manufacturer's instructions; DAB-Kit, Vector Laboratories). Sections were washed in 0.05 M Tris/HCl (pH 7.6) and again in 0.1 M PBS prior to xylol clearance, dehydration, and coverslipping with Eukitt mounting medium (Kindler). For identification of cortical layers, sections adjacent to the ones used for immunocytochemistry were stained with toluidine-blue (Sigma). Digital images of stained sections were acquired using an Axiophot II microscope (Zeiss). Final images were assembled in Corel PhotoPaint X3 and, in case of higher magnifications, were a composition of 4-5 images along the longitudinal axis of the primary visual cortex [29, 30]. Contrast and luminosity were adjusted in Corel PhotoPaint X3 for images obtained from monocularly enucleated animals.
Immunofluorescence and confocal microscopy
Antibodies used in double-labeling experiments were applied sequentially and blocking steps were performed using normal sera of the host species from which the respective secondary antibodies were derived. Cryostat sections (40 μm) of occipital lobes were rinsed in PBS before the epitope retrieval step was performed, as described above. Nonspecific antibody binding sites were blocked with 3% normal serum in PBS for 1 h at RT. Sections were then incubated with mouse monoclonal TP25.99 antibody, 1:300 in 3% normal serum in PBS, for 16 hrs at 4°C, washed, and incubated in secondary antiserum (Alexa 488-coupled goat anti-mouse, Molecular Probes, Invitrogen, Leiden, Netherlands) at a dilution of 1:500 for 4 h in a lightproof container. Sections were then washed and incubated with either rabbit anti-MAP2 antibody (1:200, Synaptic Systems, Göttingen, Germany), rabbit anti-NMDAR1 (1;200, Synaptic Systems), rabbit anti-GFAP (1:500, Synaptic Systems), or rabbit anti-vimentin antibody (1:200, Synaptic Systems) in 3% normal serum in PBS for 16 h at 4°C. For GFAP-vimentin colocalization studies, the epitope retrieval step was not performed and following primary antibodies were used: rabbit anti-vimentin (1:200, Synaptic Systems) and mouse anti-GFAP (1:200, Sigma). Sections were then washed and incubated 4 h at RT in secondary antiserum (Alexa 568-coupled goat anti-rabbit, Molecular Probes, Invitrogen) diluted 1:500 in 3% normal serum in PBS. Thereafter, sections were washed in PBS and floated/mounted on SuperFrost ®Plus slides (Menzel-Gläser GmbH, Braunschweig, Germany) in distilled water, allowed to dry overnight at 4°C, and coverslipped with mounting medium (Aqua-Polymount, Polysciences Inc., Warrington, PA, USA). For control sections, the same procedures were performed omitting the primary antibody.
Confocal microscopy was performed using a laser-scanning microscope (LSM 5 Pascal, Zeiss) with an argon 488 nm laser and a helium/neon 543 nm laser (in multiple-tracking mode). High magnification, single optical plane images of layer IV neurons of the primary visual cortex [29, 30] or of the subcortical white matter in the occipital lobes were obtained at a resolution of 1,024 × 1,024 with an Apochromat 63× oil objective (NA = 1.4) and immersion oil (Immersol, Zeiss; refractive index = 1.518). The control sections (no primary antibodies) displayed only background fluorescence (data not shown).
Brains were immediately removed from terminally anesthetized animals (see above), and the whole visual cortices were quickly dissected. Whole visual cortex samples were homogenized with a Dounce homogenizer (tight pestle) in ice-cold homogenization buffer consisting of 50 mM Tris/HCl pH 7.4, 7.5% glycerol, 150 mM NaCl, 1 mM EDTA, 1% Triton-X 100, and complete protease inhibitor cocktail (Roche Diagnostics, Mannheim, Germany). After homogenization, the samples were centrifuged at 4,000× g for 20 min at 4°C. The resulting supernatant was centrifuged again until it was clear. Protein concentration was measured using the Bio-Rad DC Protein assay (Bio-Rad Laboratories, Hercules, CA, USA).
Protein preparations were electrophoresed in 12.5% SDS gels under reducing conditions. Proteins were subsequently transferred to nitrocellulose membranes (Schleicher and Schuell, Dassel, Germany) via semidry electroblotting for 2 h at 1 mA/cm2 in transfer buffer containing 25 mM Tris-base, 150 mM glycine, and 10% (v/v) methanol. After the transfer, the blotted membranes were blocked with 5% (w/v) milk powder and 0.1% Tween-20 in PBS for 1 h at RT and were then incubated with either monoclonal TP25.99 (1:1,000) or monoclonal anti-SNAP-25 (1:1,000, Synaptic Systems) antibodies overnight at 4°C. After washing three times for 5 min in PBS/0.1% Tween-20, blots were incubated for 1 h at RT with horseradish peroxidase-coupled goat anti-mouse IgG (1:4,000, Santa Cruz Biotechnology, Santa Cruz, USA). Prior to visualization, blots were washed in PBS/0.1% Tween-20 (3 × 5 min) and once more in PBS. Signals were visualized using SuperSignal West Dura enhanced luminescence substrate (Pierce Biotechnology, Rockford, IL, USA). Membranes were subsequently stripped in a mixture of β-mercaptoethanol and SDS in PBS and incubated with monoclonal anti-β-actin antibody (1:4000, Sigma). For controls, the same procedures were performed using the mouse IgG (Sigma) instead of a primary antibody. Control IgG yielded no signals in the Western blot (data not shown). For quantification, blots were visualized with MCID Basic software (Imaging Research Inc., St. Catherines, Ontario, Canada) so that nonsaturating bands were obtained. Quantification was performed using the gel analysis plug-in of ImageJ (U.S. National Institutes of Health, Bethesda, Maryland, USA). Values obtained for MHCI and SNAP-25 were normalized to ß-actin values.
Image analysis of sections from monocularly enucleated animals
Digital images of immunostained tissue sections were acquired using an Axiophot II microscope (Zeiss). Images were taken from the V1 areas of right visual cortices. Variations in MHCI immunoreactivity through layer IV were measured as described previously . Briefly, images were normalized and black-white inverted using ImageJ (U.S. National Institutes of Health, Bethesda, MD, USA) and regions within layer IV that encompassed the thickness of the entire layer IV (2 mm in length and approximately 1 mm in thickness) were defined as the regions of interest. For identification of cortical layers, sections adjacent to the ones used for immunocytochemistry were stained with toluidine-blue (Sigma). The density profile of layer IV in the primary visual cortex (region demarcated according to [29, 30]) was obtained using ImageJ. Values were averaged (using the four closest neighbors) and plotted as a function of the distance along layer IV parallel to the pia mater. After delineating the borders of immunoreactive patches, their width was measured using ImageJ and compared using Student's t-test (GraphPad Prism version 4 for Windows).
Expression of MHCI molecules in LGN and V1 of the common marmoset
Expression levels of MHCI molecules are regulated by neuronal activity
Neurons with higher MHCI-HC expression receive afferents from the intact eye
Several recent studies have implicated MHCI proteins in the elimination of excess synapses in the visual system during distinct phases of cortical development [12, 15, 23]. MHCI molecules, as well as the organization of the visual system, differ between rodents and primates, including humans [17, 24]. We therefore investigated the expression of neuronal MHCI molecules in the brain of the common marmoset monkey. To the best of our knowledge, the present study is the first to characterize the spatio-temporal pattern of MHCI gene expression in the primary visual cortex during postnatal development in a nonhuman primate.
Expression and localization of MHCI molecules in V1
It is known that virtually all nucleated cells express MHCI molecules in form of completely assembled heterotrimers, and in some cases in form of the peptide-free heavy chains [4, 6]. The present study clearly showed that MHCI molecules are expressed on MAP-2 positive neurons of V1. However, in this and our previous studies [56, 57], we were only able to detect neuronal expression of MHCI heavy chain proteins. In addition, in situ hybridization and immunohistochemistry revealed high expression levels of MHCI-HC throughout the subcortical white matter (SWM) of the occipital lobes, consistent with what has previously been observed in cats . In SWM, MHCI-HC protein colocalized with vimentin-positive radial glial cells. These highly specialized cells are regarded as neuronal precursors and are involved in proper development and migration of cortical neurons . In primates, these cells are also GFAP-positive from early prenatal stages, but can be distinguished from astrocytes on the basis of their distinct morphology and localization . We did not detect any vimentin-positive cells in the cortical layers, and MHCI-HC was not expressed in GFAP-positive cortical astrocytes (Additional file 4), although these astrocytes were stained with the antibody that recognized fully assembled, heterotrimeric form of MHCI (Additional file 5). These findings further implicate MHCI in proper development of the visual cortex, although their potential function in this context remains elusive . As the marmoset MHCI gene cluster is not fully characterized yet, we hope that future studies will shed light on the exact identity and potential functional differences between neuronal, radial glial and microglial MHCI.
Previous studies have shown that MHCI proteins may be localized to both pre- and postsynaptic sites [14, 23, 57, 73, 74]. The MHCI-HC molecules detected here with confocal microscopy and TP25.99 antibody seem to be associated with the postsynaptic sites (cell bodies and dendrites, Figure 5) and they displayed no obvious preference for excitatory vs inhibitory synapses (Additional file 3), as has previously been reported .
MHCI-HC mRNA and protein expression in the developing visual cortex
In contrast to the previous studies in rodents and cats [12, 15], we did not detect expression of MHCI-HC in the LGN at postnatal stages used in this study. It is possible that MHCI genes are expressed prenatally in the marmoset LGN, as the segregation of LGN layers in primates occurs in utero[39, 40]. Furthermore, the probe used here for the detection of MHCI-HC transcripts is specific for a subset of MHCI molecules, while the probes used in previous studies were mostly pan-specific and therefore detected a larger variety of MHCI molecules . Even so, the present data showed that MHCI genes are strongly expressed throughout all regions of the visual cortex of the marmoset. In contrast to the in situ hybridization that revealed a distinct spatio-temporal pattern of MHCI-HC expression, the staining pattern generated with the TP25.99 antibody was rather uniform throughout all cortical layers and developmental stages. While the MHCI subset-specific riboprobe used for in situ hybridization very likely targeted only a few MHCI genes, the antibody we used for histochemical detection of MHCI targets the most conserved, monomorphic part of these molecules. Hence, the TP25.99 antibody likely recognized a large number of MHCI-HC proteins expressed on V1 neurons, including the ones targeted at the mRNA level by in situ hybridization riboprobe. As previously mentioned, the common marmoset MHCI gene cluster is not yet fully characterized and so far only two loci have been described, the classical Caja-G and the non-classical Caja-E . Based on this (Additional file 2) and our previous studies [56, 57], we believe that the neuronal MHCI-HC detected in the present study is a classical marmoset MHCI, Caja-G. Nonetheless, the levels of MHCI-HC protein expression in the visual cortex correlated well with the levels of SNAP-25 (synaptosome-associated protein 25 kDa). This protein is an established synaptogenesis marker [43, 75, 76] and the present data on SNAP-25 protein expression levels confirmed previous findings that synapse density in the marmoset V1 reaches very high values in the third postnatal month, followed by a rapid decline due to synapse elminiation [25, 26]. Contrary to our expectations and to the proposed role for MHCI in the elimination of synapses [12, 15, 23], MHCI-HC protein expression was at its peak already in the third postnatal month, together with SNAP-25 levels, and was in decline in the fifth postnatal month. This suggests that, as recently proposed , MHCI proteins may exert their function during synaptogenesis, rather than during refinement and elimination of synapses.
MHCI expression in the visual cortex is activity dependent
As genetic manipulation of primates is still either unavailable or at an early stage , we used monocular deprivation as an alternative model to further clarify the role of MHCI molecules in the marmoset visual cortex. Prolonged monocular deprivation induced by various means (in the order of months) induces morphological changes in the visual cortex because neurons that receive input from the intact eye expand their connections and cortical space at the expense of neurons that receive afferents from the deprived eye [21, 60]. A number of studies have shown that monocular deprivation results in the upregulation of growth factors and genes associated with neuronal degeneration or synaptogenesis [64, 65, 78]. In mice, monocular deprivation increases levels of SNAP-25 and SynCAM (synaptic cell adhesion molecule) transcripts , both of which are required for synaptogenesis [79–81]. Interestingly, MHCI-HC transcript levels detected in the present study after monocular enucleation with qRT-PCR and in situ hybridization for classical marmoset MHCI-HC were also higher in response to monocular enucleation. The levels of neuronal activity marker c-Fos were also elevated. On the contrary, GFAP displayed no significant change in either expression levels or in expression pattern in enucleated animals (Additional file 9). This even further correlated MHCI-HC levels with neuronal activity, and not with microglial activation.
We used immunohistochemistry to localize the elevated MHCI-HC levels to neurons receiving afferent inputs from the intact eye. In V1 of enucleated animals, both the well-established activity marker c-Fos [68, 69] and MHCI-HC immunostaining revealed a pattern of column-like patches, where regions of high c-Fos and high MHCI-HC immunoreactivity overlapped and were wider than regions with low immunoreactivity. These changes were visible not only in layer IV, but also in layers I-III and layer VI, which is not surprising since it is known that prolonged deprivation causes responses in neurons in all cortical layers . Previous studies in marmosets have shown that such patches correspond to ocular dominance columns. They differ in size in monocularly deprived animals, with wider ODCs receiving input from the intact eye and showing higher levels of NMDAR1 [61, 70]. Interestingly, MHCI-HC protein detected in this study also localized to NMDAR1-positive neurons in the cortex of enucleated animals (Additional file 7), which is also indicative of MHCI association with neuronal activity [61, 70]. Therefore, MHCI-HC immunoreactivity in the visual cortex of enucleated animals suggested the upregulation of expression of this class of genes in neurons receiving input from the intact eye. As mentioned above, synaptogenesis in the marmoset visual cortex peaks in the third postnatal month. Although elimination of synapses and synaptogenesis may occur within the same time window in the developing V1 , it is interesting to note that MHCI expression peaks during the synaptogenesis stage. Moreover, the observed upregulation of MHCI levels in response to monocular enucleation points to a possible role in synaptogenesis.
Potential interspecies differences in properties of neuronal MHCI molecules
The so-called classical MHCI molecules detected in this study are traditionally regarded as having only immunity-related functions, but they have recently been implicated in synaptic refinement in the visual system . Even though our data indicated a potential interspecies difference in the function of classical MHCI in the visual system, the very large number of these genes and the fact that there are no true orthologues between rodents and primates  makes direct comparisons very difficult. On the other hand, the possible interspecies differences in function are not surprising. MHCI molecules exhibit a multitude of functions and may, for example, transmit both activating and inhibitory signals in the immune system [7, 84]. MHCI proteins are also necessary for immunological synapse formation . In addition to their role in synaptic refinement and stripping, MHCI molecules have been implicated in stabilization of certain types of terminals in the peripheral nervous system of mice . The exact mechanism and role of MHCI in the visual cortex is yet to be elucidated, although previous studies have shown that MHCI may associate with PI-3, which is known to be a synaptogenic molecule [8, 87]. Another possibility includes the MHCI-presenting peptides, as it is known that they reflect the metabolic state of the cell . Given that neuronal MHCI expression in the visual cortex is activity-regulated, similar to c-Fos ( and this study), this would enable it to mark or tag neurons or neuronal populations that display a distinct activity. A recent study also suggested that neuronal MHCI may be involved in NMDA-induced internalization of AMPARs (α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate receptors, ). AMPARs belong to the group of activity-regulated molecules involved in developmental plasticity in the visual cortex [90, 91]. As we were able to reliably detect only the free heavy chain form of MHCI proteins on neurons (this study and [56, 57]), the suggested link between MHCI and trafficking of AMPARs is very intriguing considering our current study and previous studies on MHCI involvement in receptor trafficking [6, 56, 57].
As previously mentioned, there are no true orthologues between MHC class I genes of rodents and primates . MHC gene cluster shows significant interspecies variability, as well as a fast evolutionary rate [2, 3, 17]. This makes drawing parallels between rodent and primate studies extremely difficult. Furthermore, current policies on the use of non-human primates in research are very limiting, which makes functional studies on non-human primates hard to perform.
It is our hope that the further development of transgenic marmoset models , more flexible research policies, as well as detailed characterization of the marmoset MHC cluster, will provide future researchers with the tools to investigate the potential functional differences between neuronal MHCI molecules in rodents and primates in more detail.
Despite the possible interspecies differences in expression of MHCI molecules in the brain, all studies on this topic have highlighted yet another important role of these molecules. MHC genes have been linked to a number of CNS disorders and the discovery of their roles in plasticity processes in the brain has shed new light on their etiology [84, 92–94]. Further research is warranted to elucidate the exact mechanisms of action of the neuronal MHCI genes and the exact identity of the neuronal MHCI gene possibly involved in synaptogenesis.
The authors wish to thank T. Meyer-Burhenne and S. Leineweber for expert animal care and A. Hoffmann, J. Krenzek and A. Heutz for technical assistance. We also wish to thank S. Ferrone for providing the TP25.99 and Q1/28 antibodies, B. Uchanska-Ziegler and C. Rosner for providing the W6/32 antibody and N. Yee for critical reading of the manuscript. AR was funded by NEUREST MEST-CT-2004-504193 and GGNB/IMPRS Molecular Biology Göttingen.
- Kelley J, Trowsdale J: Features of MHC and NK gene clusters. Transpl Immunol. 2005, 14: 129-134. 10.1016/j.trim.2005.03.001.View ArticlePubMedGoogle Scholar
- Gunther E, Walter L: The major histocompatibility complex of the rat (Rattus norvegicus). Immunogenetics. 2001, 53: 520-542. 10.1007/s002510100361.View ArticlePubMedGoogle Scholar
- Kaufman J, Salomonsen J, Flajnik M: Evolutionary conservation of MHC class I and class II molecules--different yet the same. Semin Immunol. 1994, 6: 411-424. 10.1006/smim.1994.1050.View ArticlePubMedGoogle Scholar
- Solheim JC: Class I MHC molecules: assembly and antigen presentation. Immunol Rev. 1999, 172: 11-19. 10.1111/j.1600-065X.1999.tb01352.x.View ArticlePubMedGoogle Scholar
- Cresswell P, Ackerman AL, Giodini A, Peaper DR, Wearsch PA: Mechanisms of MHC class I-restricted antigen processing and cross-presentation. Immunol Rev. 2005, 207: 145-157. 10.1111/j.0105-2896.2005.00316.x.View ArticlePubMedGoogle Scholar
- Arosa FA, Santos SG, Powis SJ: Open conformers: the hidden face of MHC-I molecules. Trends Immunol. 2007, 28: 115-123. 10.1016/j.it.2007.01.002.View ArticlePubMedGoogle Scholar
- Fishman D, Elhyany S, Segal S: Non-immune functions of MHC class I glycoproteins in normal and malignant cells. Folia Biol (Praha). 2004, 50: 35-42.Google Scholar
- Ramalingam TS, Chakrabarti A, Edidin M: Interaction of class I human leukocyte antigen (HLA-I) molecules with insulin receptors and its effect on the insulin-signaling cascade. Mol Biol Cell. 1997, 8: 2463-2474.PubMed CentralView ArticlePubMedGoogle Scholar
- Aarli JA: The immune system and the nervous system. J Neurol. 1983, 229: 137-154. 10.1007/BF00313738.View ArticlePubMedGoogle Scholar
- Linda H, Hammarberg H, Cullheim S, Levinovitz A, Khademi M, Olsson T: Expression of MHC class I and beta2-microglobulin in rat spinal motoneurons: regulatory influences by IFN-gamma and axotomy. Exp Neurol. 1998, 150: 282-295. 10.1006/exnr.1997.6768.View ArticlePubMedGoogle Scholar
- Neumann H, Cavalie A, Jenne DE, Wekerle H: Induction of MHC class I genes in neurons. Science. 1995, 269: 549-552. 10.1126/science.7624779.View ArticlePubMedGoogle Scholar
- Huh GS, Boulanger LM, Du H, Riquelme PA, Brotz TM, Shatz CJ: Functional requirement for class I MHC in CNS development and plasticity. Science. 2000, 290: 2155-2159. 10.1126/science.290.5499.2155.PubMed CentralView ArticlePubMedGoogle Scholar
- McConnell MJ, Huang YH, Datwani A, Shatz CJ: H2-K(b) and H2-D(b) regulate cerebellar long-term depression and limit motor learning. Proc Natl Acad Sci USA. 2009, 106: 6784-6789. 10.1073/pnas.0902018106.PubMed CentralView ArticlePubMedGoogle Scholar
- Goddard CA, Butts DA, Shatz CJ: Regulation of CNS synapses by neuronal MHC class I. Proc Natl Acad Sci USA. 2007, 104: 6828-6833. 10.1073/pnas.0702023104.PubMed CentralView ArticlePubMedGoogle Scholar
- Corriveau RA, Huh GS, Shatz CJ: Regulation of class I MHC gene expression in the developing and mature CNS by neural activity. Neuron. 1998, 21: 505-520. 10.1016/S0896-6273(00)80562-0.View ArticlePubMedGoogle Scholar
- Boulanger LM: Immune proteins in brain development and synaptic plasticity. Neuron. 2009, 64: 93-109. 10.1016/j.neuron.2009.09.001.View ArticlePubMedGoogle Scholar
- Kumanovics A, Takada T, Lindahl KF: Genomic organization of the mammalian MHC. Annu Rev Immunol. 2003, 21: 629-657. 10.1146/annurev.immunol.21.090501.080116.View ArticlePubMedGoogle Scholar
- Sur M, Rubenstein JL: Patterning and plasticity of the cerebral cortex. Science. 2005, 310: 805-810. 10.1126/science.1112070.View ArticlePubMedGoogle Scholar
- Shatz CJ: Emergence of order in visual system development. Proc Natl Acad Sci USA. 1996, 93: 602-608. 10.1073/pnas.93.2.602.PubMed CentralView ArticlePubMedGoogle Scholar
- Katz LC, Crowley JC: Development of cortical circuits: lessons from ocular dominance columns. Nat Rev Neurosci. 2002, 3: 34-42. 10.1038/nrn703.View ArticlePubMedGoogle Scholar
- Shatz CJ, Stryker MP: Ocular dominance in layer IV of the cat's visual cortex and the effects of monocular deprivation. J Physiol. 1978, 281: 267-283.PubMed CentralView ArticlePubMedGoogle Scholar
- Berardi N, Pizzorusso T, Ratto GM, Maffei L: Molecular basis of plasticity in the visual cortex. Trends Neurosci. 2003, 26: 369-378. 10.1016/S0166-2236(03)00168-1.View ArticlePubMedGoogle Scholar
- Datwani A, McConnell MJ, Kanold PO, Micheva KD, Busse B, Shamloo M, Smith SJ, Shatz CJ: Classical MHCI molecules regulate retinogeniculate refinement and limit ocular dominance plasticity. Neuron. 2009, 64: 463-470. 10.1016/j.neuron.2009.10.015.PubMed CentralView ArticlePubMedGoogle Scholar
- Antonini A, Fagiolini M, Stryker MP: Anatomical correlates of functional plasticity in mouse visual cortex. J Neurosci. 1999, 19: 4388-4406.PubMed CentralPubMedGoogle Scholar
- Missler M, Wolff JR, Rothe H, Heger W, Merker HJ, Treiber A, Scheid R, Crook GA: Developmental biology of the common marmoset: proposal for a "postnatal staging". J Med Primatol. 1992, 21: 285-298.PubMedGoogle Scholar
- Missler M, Eins S, Merker HJ, Rothe H, Wolff JR: Pre- and postnatal development of the primary visual cortex of the common marmoset. I. A changing space for synaptogenesis. J Comp Neurol. 1993, 333: 41-52. 10.1002/cne.903330104.View ArticlePubMedGoogle Scholar
- Brass W, Schebitz H: Operationen an Hund und Katze. 2007, Stuttgart: MVS Medizienverlage Stuttgart, 2007Google Scholar
- DeBruyn EJ, Casagrande VA: Demonstration of ocular dominance columns in a New World primate by means of monocular deprivation. Brain Res. 1981, 207: 453-458. 10.1016/0006-8993(81)90378-4.View ArticlePubMedGoogle Scholar
- Fritsches KA, Rosa MG: Visuotopic organisation of striate cortex in the marmoset monkey (Callithrix jacchus). J Comp Neurol. 1996, 372: 264-282. 10.1002/(SICI)1096-9861(19960819)372:2<264::AID-CNE8>3.0.CO;2-1.View ArticlePubMedGoogle Scholar
- Rosa MG, Fritsches KA, Elston GN: The second visual area in the marmoset monkey: visuotopic organisation, magnification factors, architectonical boundaries, and modularity. J Comp Neurol. 1997, 387: 547-567. 10.1002/(SICI)1096-9861(19971103)387:4<547::AID-CNE6>3.0.CO;2-2.View ArticlePubMedGoogle Scholar
- Rozen S, Skaletsky H: Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol. 2000, 132: 365-386.PubMedGoogle Scholar
- Abumaria N, Ribic A, Anacker C, Fuchs E, Flugge G: Stress upregulates TPH1 but not TPH2 mRNA in the rat dorsal raphe nucleus: identification of two TPH2 mRNA splice variants. Cell Mol Neurobiol. 2008, 28: 331-342. 10.1007/s10571-007-9259-5.View ArticlePubMedGoogle Scholar
- Ririe KM, Rasmussen RP, Wittwer CT: Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal Biochem. 1997, 245: 154-160. 10.1006/abio.1996.9916.View ArticlePubMedGoogle Scholar
- Tanabe M, Sekimata M, Ferrone S, Takiguchi M: Structural and functional analysis of monomorphic determinants recognized by monoclonal antibodies reacting with the HLA class I alpha 3 domain. J Immunol. 1992, 148: 3202-3209.PubMedGoogle Scholar
- D'Urso CM, Wang ZG, Cao Y, Tatake R, Zeff RA, Ferrone S: Lack of HLA class I antigen expression by cultured melanoma cells FO-1 due to a defect in B2 m gene expression. J Clin Invest. 1991, 87: 284-292.PubMed CentralView ArticlePubMedGoogle Scholar
- Barnstable CJ, Bodmer WF, Brown G, Galfre G, Milstein C, Williams AF, Ziegler A: Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell. 1978, 14: 9-20. 10.1016/0092-8674(78)90296-9.View ArticlePubMedGoogle Scholar
- Catalano SM, Chang CK, Shatz CJ: Activity-dependent regulation of NMDAR1 immunoreactivity in the developing visual cortex. J Neurosci. 1997, 17: 8376-8390.PubMedGoogle Scholar
- Cadavid LF, Shufflebotham C, Ruiz FJ, Yeager M, Hughes AL, Watkins DI: Evolutionary instability of the major histocompatibility complex class I loci in New World primates. Proc Natl Acad Sci USA. 1997, 94: 14536-14541. 10.1073/pnas.94.26.14536.PubMed CentralView ArticlePubMedGoogle Scholar
- Rakic P: Prenatal genesis of connections subserving ocular dominance in the rhesus monkey. Nature. 1976, 261: 467-471. 10.1038/261467a0.View ArticlePubMedGoogle Scholar
- Rakic P: Prenatal development of the visual system in rhesus monkey. Philos Trans R Soc Lond B Biol Sci. 1977, 278: 245-260. 10.1098/rstb.1977.0040.View ArticlePubMedGoogle Scholar
- Fayen J, Huang JH, Ferrone S, Tykocinski ML: Negative signaling by anti-HLA class I antibodies is dependent upon two triggering events. Int Immunol. 1998, 10: 1347-1358. 10.1093/intimm/10.9.1347.View ArticlePubMedGoogle Scholar
- Desai SA, Wang X, Noronha EJ, Zhou Q, Rebmann V, Grosse-Wilde H, Moy FJ, Powers R, Ferrone S: Structural relatedness of distinct determinants recognized by monoclonal antibody TP25.99 on beta 2-microglobulin-associated and beta 2-microglobulin-free HLA class I heavy chains. J Immunol. 2000, 165: 3275-3283.View ArticlePubMedGoogle Scholar
- Sidor-Kaczmarek J, Labuda C, Litwinowicz B, Spodnik JH, Kowianski P, Dziewiatkowski J, Morys J: Developmental expression of SNAP-25 protein in the rat striatum and cerebral cortex. Folia Morphol (Warsz). 2004, 63: 285-288.Google Scholar
- Harada A, Teng J, Takei Y, Oguchi K, Hirokawa N: MAP2 is required for dendrite elongation, PKA anchoring in dendrites, and proper PKA signal transduction. J Cell Biol. 2002, 158: 541-549. 10.1083/jcb.200110134.PubMed CentralView ArticlePubMedGoogle Scholar
- Shiomura Y, Hirokawa N: Colocalization of microtubule-associated protein 1A and microtubule-associated protein 2 on neuronal microtubules in situ revealed with double-label immunoelectron microscopy. J Cell Biol. 1987, 104: 1575-1578. 10.1083/jcb.104.6.1575.View ArticlePubMedGoogle Scholar
- Cooper B, Werner HB, Flugge G: Glycoprotein M6a is present in glutamatergic axons in adult rat forebrain and cerebellum. Brain Res. 2008, 1197: 1-12. 10.1016/j.brainres.2007.11.066.View ArticlePubMedGoogle Scholar
- Muller BM, Kistner U, Kindler S, Chung WJ, Kuhlendahl S, Fenster SD, Lau LF, Veh RW, Huganir RL, Gundelfinger ED, Garner CC: SAP102, a novel postsynaptic protein that interacts with NMDA receptor complexes in vivo. Neuron. 1996, 17: 255-265. 10.1016/S0896-6273(00)80157-9.View ArticlePubMedGoogle Scholar
- Lau LF, Mammen A, Ehlers MD, Kindler S, Chung WJ, Garner CC, Huganir RL: Interaction of the N-methyl-D-aspartate receptor complex with a novel synapse-associated protein, SAP102. J Biol Chem. 1996, 271: 21622-21628. 10.1074/jbc.271.35.21622.View ArticlePubMedGoogle Scholar
- Kirsch J, Betz H: Widespread expression of gephyrin, a putative glycine receptor-tubulin linker protein, in rat brain. Brain Res. 1993, 621: 301-310. 10.1016/0006-8993(93)90120-C.View ArticlePubMedGoogle Scholar
- Kirsch J, Betz H: The postsynaptic localization of the glycine receptor-associated protein gephyrin is regulated by the cytoskeleton. J Neurosci. 1995, 15: 4148-4156.PubMedGoogle Scholar
- Campbell K, Gotz M: Radial glia: multi-purpose cells for vertebrate brain development. Trends Neurosci. 2002, 25: 235-238. 10.1016/S0166-2236(02)02156-2.View ArticlePubMedGoogle Scholar
- Mo Z, Moore AR, Filipovic R, Ogawa Y, Kazuhiro I, Antic SD, Zecevic N: Human cortical neurons originate from radial glia and neuron-restricted progenitors. J Neurosci. 2007, 27: 4132-4145. 10.1523/JNEUROSCI.0111-07.2007.View ArticlePubMedGoogle Scholar
- McDermott KW, Lantos PL: The distribution of glial fibrillary acidic protein and vimentin in postnatal marmoset (Callithrix jacchus) brain. Brain Res Dev Brain Res. 1989, 45: 169-177. 10.1016/0165-3806(89)90036-9.View ArticlePubMedGoogle Scholar
- Pecchi E, Dallaporta M, Charrier C, Pio J, Jean A, Moyse E, Troadec JD: Glial fibrillary acidic protein (GFAP)-positive radial-like cells are present in the vicinity of proliferative progenitors in the nucleus tractus solitarius of adult rat. J Comp Neurol. 2007, 501: 353-368. 10.1002/cne.21259.View ArticlePubMedGoogle Scholar
- Jefferies WA, MacPherson GG: Expression of the W6/32 HLA epitope by cells of rat, mouse, human and other species: critical dependence on the interaction of specific MHC heavy chains with human or bovine beta 2-microglobulin. Eur J Immunol. 1987, 17: 1257-1263. 10.1002/eji.1830170907.View ArticlePubMedGoogle Scholar
- Rolleke U, Flugge G, Plehm S, Schlumbohm C, Armstrong VW, Dressel R, Uchanska-Ziegler B, Ziegler A, Fuchs E, Czeh B, Walter L: Differential expression of major histocompatibility complex class I molecules in the brain of a New World monkey, the common marmoset (Callithrix jacchus). J Neuroimmunol. 2006, 176: 39-50. 10.1016/j.jneuroim.2006.04.015.View ArticlePubMedGoogle Scholar
- Ribic A, Zhang M, Schlumbohm C, Matz-Rensing K, Uchanska-Ziegler B, Flugge G, Zhang W, Walter L, Fuchs E: Neuronal MHC class I molecules are involved in excitatory synaptic transmission at the hippocampal mossy fiber synapses of marmoset monkeys. Cell Mol Neurobiol. 2010, 30: 827-839. 10.1007/s10571-010-9510-3.PubMed CentralView ArticlePubMedGoogle Scholar
- McAllister AK: Cellular and molecular mechanisms of dendrite growth. Cereb Cortex. 2000, 10: 963-973. 10.1093/cercor/10.10.963.View ArticlePubMedGoogle Scholar
- Antonini A, Gillespie DC, Crair MC, Stryker MP: Morphology of single geniculocortical afferents and functional recovery of the visual cortex after reverse monocular deprivation in the kitten. J Neurosci. 1998, 18: 9896-9909.PubMed CentralPubMedGoogle Scholar
- Hubel DH, Wiesel TN, LeVay S: Plasticity of ocular dominance columns in monkey striate cortex. Philos Trans R Soc Lond B Biol Sci. 1977, 278: 377-409. 10.1098/rstb.1977.0050.View ArticlePubMedGoogle Scholar
- Fonta C, Chappert C, Imbert M: Effect of monocular deprivation on NMDAR1 immunostaining in ocular dominance columns of the marmoset Callithrix jacchus. Vis Neurosci. 2000, 17: 345-352. 10.1017/S0952523800173031.View ArticlePubMedGoogle Scholar
- Antonini A, Stryker MP: Effect of sensory disuse on geniculate afferents to cat visual cortex. Vis Neurosci. 1998, 15: 401-409. 10.1017/S0952523898153105.PubMed CentralView ArticlePubMedGoogle Scholar
- Schmidt KE, Stephan M, Singer W, Lowel S: Spatial analysis of ocular dominance patterns in monocularly deprived cats. Cereb Cortex. 2002, 12: 783-796. 10.1093/cercor/12.8.783.View ArticlePubMedGoogle Scholar
- Lachance PE, Chaudhuri A: Microarray analysis of developmental plasticity in monkey primary visual cortex. J Neurochem. 2004, 88: 1455-1469. 10.1046/j.1471-4159.2003.02274.x.View ArticlePubMedGoogle Scholar
- Tropea D, Kreiman G, Lyckman A, Mukherjee S, Yu H, Horng S, Sur M: Gene expression changes and molecular pathways mediating activity-dependent plasticity in visual cortex. Nat Neurosci. 2006, 9: 660-668. 10.1038/nn1689.View ArticlePubMedGoogle Scholar
- Benson DL, Isackson PJ, Gall CM, Jones EG: Differential effects of monocular deprivation on glutamic acid decarboxylase and type II calcium-calmodulin-dependent protein kinase gene expression in the adult monkey visual cortex. J Neurosci. 1991, 11: 31-47.PubMedGoogle Scholar
- Duffy KR, Livingstone MS: Loss of neurofilament labeling in the primary visual cortex of monocularly deprived monkeys. Cereb Cortex. 2005, 15: 1146-1154. 10.1093/cercor/bhh214.PubMed CentralView ArticlePubMedGoogle Scholar
- Soares JG, Pereira AC, Botelho EP, Pereira SS, Fiorani M, Gattass R: Differential expression of Zif268 and c-Fos in the primary visual cortex and lateral geniculate nucleus of normal Cebus monkeys and after monocular lesions. J Comp Neurol. 2005, 482: 166-175. 10.1002/cne.20361.View ArticlePubMedGoogle Scholar
- Van der Gucht E, Hof PR, Van Brussel L, Burnat K, Arckens L: Neurofilament protein and neuronal activity markers define regional architectonic parcellation in the mouse visual cortex. Cereb Cortex. 2007, 17: 2805-2819. 10.1093/cercor/bhm012.View ArticlePubMedGoogle Scholar
- Fonta C, Chappert C, Imbert M: N-methyl-D-aspartate subunit R1 involvement in the postnatal organization of the primary visual cortex of Callithrix jacchus. J Comp Neurol. 1997, 386: 260-276. 10.1002/(SICI)1096-9861(19970922)386:2<260::AID-CNE7>3.0.CO;2-#.View ArticlePubMedGoogle Scholar
- Levitt P, Rakic P: Immunoperoxidase localization of glial fibrillary acidic protein in radial glial cells and astrocytes of the developing rhesus monkey brain. J Comp Neurol. 1980, 193: 815-840. 10.1002/cne.901930316.View ArticlePubMedGoogle Scholar
- Laguna Goya R, Tyers P, Barker RA: Adult neurogenesis is unaffected by a functional knock-out of MHC class I in mice. Neuroreport. 2010, 21: 349-353. 10.1097/WNR.0b013e3283377463.View ArticlePubMedGoogle Scholar
- Thams S, Brodin P, Plantman S, Saxelin R, Karre K, Cullheim S: Classical major histocompatibility complex class I molecules in motoneurons: new actors at the neuromuscular junction. J Neurosci. 2009, 29: 13503-13515. 10.1523/JNEUROSCI.0981-09.2009.View ArticlePubMedGoogle Scholar
- Needleman LA, Liu XB, El-Sabeawy F, Jones EG, McAllister AK: MHC class I molecules are present both pre- and postsynaptically in the visual cortex during postnatal development and in adulthood. Proc Natl Acad Sci USA. 2010, 107: 16999-17004. 10.1073/pnas.1006087107.PubMed CentralView ArticlePubMedGoogle Scholar
- Oyler GA, Polli JW, Wilson MC, Billingsley ML: Developmental expression of the 25-kDa synaptosomal-associated protein (SNAP-25) in rat brain. Proc Natl Acad Sci USA. 1991, 88: 5247-5251. 10.1073/pnas.88.12.5247.PubMed CentralView ArticlePubMedGoogle Scholar
- Gingras J, Cabana T: Synaptogenesis in the brachial and lumbosacral enlargements of the spinal cord in the postnatal opossum, Monodelphis domestica. J Comp Neurol. 1999, 414: 551-560. 10.1002/(SICI)1096-9861(19991129)414:4<551::AID-CNE9>3.0.CO;2-W.View ArticlePubMedGoogle Scholar
- Sasaki E, Suemizu H, Shimada A, Hanazawa K, Oiwa R, Kamioka M, Tomioka I, Sotomaru Y, Hirakawa R, Eto T: Generation of transgenic non-human primates with germline transmission. Nature. 2009, 459: 523-527. 10.1038/nature08090.View ArticlePubMedGoogle Scholar
- Lyckman AW, Horng S, Leamey CA, Tropea D, Watakabe A, Van Wart A, McCurry C, Yamamori T, Sur M: Gene expression patterns in visual cortex during the critical period: synaptic stabilization and reversal by visual deprivation. Proc Natl Acad Sci USA. 2008, 105: 9409-9414. 10.1073/pnas.0710172105.PubMed CentralView ArticlePubMedGoogle Scholar
- Osen-Sand A, Catsicas M, Staple JK, Jones KA, Ayala G, Knowles J, Grenningloh G, Catsicas S: Inhibition of axonal growth by SNAP-25 antisense oligonucleotides in vitro and in vivo. Nature. 1993, 364: 445-448. 10.1038/364445a0.View ArticlePubMedGoogle Scholar
- Biederer T, Sara Y, Mozhayeva M, Atasoy D, Liu X, Kavalali ET, Sudhof TC: SynCAM, a synaptic adhesion molecule that drives synapse assembly. Science. 2002, 297: 1525-1531. 10.1126/science.1072356.View ArticlePubMedGoogle Scholar
- Sara Y, Biederer T, Atasoy D, Chubykin A, Mozhayeva MG, Sudhof TC, Kavalali ET: Selective capability of SynCAM and neuroligin for functional synapse assembly. J Neurosci. 2005, 25: 260-270. 10.1523/JNEUROSCI.3165-04.2005.View ArticlePubMedGoogle Scholar
- Thompson I: Cortical development: Binocular plasticity turned outside-in. Curr Biol. 2000, 10: R348-350. 10.1016/S0960-9822(00)00464-4.View ArticlePubMedGoogle Scholar
- Missler M, Wolff A, Merker HJ, Wolff JR: Pre- and postnatal development of the primary visual cortex of the common marmoset. II. Formation, remodelling, and elimination of synapses as overlapping processes. J Comp Neurol. 1993, 333: 53-67. 10.1002/cne.903330105.View ArticlePubMedGoogle Scholar
- Escande-Beillard N, Washburn L, Zekzer D, Wu ZP, Eitan S, Ivkovic S, Lu Y, Dang H, Middleton B, Bilousova TV: Neurons preferentially respond to self-MHC class I allele products regardless of peptide presented. J Immunol. 2009, 184: 816-823. 10.4049/jimmunol.0902159.PubMed CentralView ArticlePubMedGoogle Scholar
- Fooksman DR, Vardhana S, Vasiliver-Shamis G, Liese J, Blair DA, Waite J, Sacristan C, Victora GD, Zanin-Zhorov A, Dustin ML: Functional anatomy of T cell activation and synapse formation. Annu Rev Immunol. 28: 79-105. 10.1146/annurev-immunol-030409-101308.
- Oliveira AL, Thams S, Lidman O, Piehl F, Hokfelt T, Karre K, Linda H, Cullheim S: A role for MHC class I molecules in synaptic plasticity and regeneration of neurons after axotomy. Proc Natl Acad Sci USA. 2004, 101: 17843-17848. 10.1073/pnas.0408154101.PubMed CentralView ArticlePubMedGoogle Scholar
- Martin-Pena A, Acebes A, Rodriguez JR, Sorribes A, de Polavieja GG, Fernandez-Funez P, Ferrus A: Age-independent synaptogenesis by phosphoinositide 3 kinase. J Neurosci. 2006, 26: 10199-10208. 10.1523/JNEUROSCI.1223-06.2006.View ArticlePubMedGoogle Scholar
- Fortier MH, Caron E, Hardy MP, Voisin G, Lemieux S, Perreault C, Thibault P: The MHC class I peptide repertoire is molded by the transcriptome. J Exp Med. 2008, 205: 595-610. 10.1084/jem.20071985.PubMed CentralView ArticlePubMedGoogle Scholar
- Fourgeaud L, Davenport CM, Tyler CM, Cheng TT, Spencer MB, Boulanger LM: MHC class I modulates NMDA receptor function and AMPA receptor trafficking. Proc Natl Acad Sci USA. 2010, 107: 22278-22283. 10.1073/pnas.0914064107.PubMed CentralView ArticlePubMedGoogle Scholar
- Heynen AJ, Yoon BJ, Liu CH, Chung HJ, Huganir RL, Bear MF: Molecular mechanism for loss of visual cortical responsiveness following brief monocular deprivation. Nat Neurosci. 2003, 6: 854-862. 10.1038/nn1100.View ArticlePubMedGoogle Scholar
- Wong-Riley MT, Jacobs P: AMPA glutamate receptor subunit 2 in normal and visually deprived macaque visual cortex. Vis Neurosci. 2002, 19: 563-573. 10.1017/S0952523802195022.View ArticlePubMedGoogle Scholar
- Sandbrink R, Hartmann T, Masters CL, Beyreuther K: Genes contributing to Alzheimer's disease. Mol Psychiatry. 1996, 1: 27-40.PubMedGoogle Scholar
- Listi F, Candore G, Balistreri CR, Grimaldi MP, Orlando V, Vasto S, Colonna-Romano G, Lio D, Licastro F, Franceschi C, Caruso C: Association between the HLA-A2 allele and Alzheimer disease. Rejuvenation Res. 2006, 9: 99-101. 10.1089/rej.2006.9.99.View ArticlePubMedGoogle Scholar
- Torres AR, Sweeten TL, Cutler A, Bedke BJ, Fillmore M, Stubbs EG, Odell D: The association and linkage of the HLA-A2 class I allele with autism. Hum Immunol. 2006, 67: 346-351. 10.1016/j.humimm.2006.01.001.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.