- Open Access
Effect of parsley (Petroselinum crispum, Apiaceae) juice against cadmium neurotoxicity in albino mice (Mus Musculus)
Behavioral and Brain Functionsvolume 12, Article number: 6 (2016)
Parsley was employed as an experimental probe to prevent the behavioral, biochemical and morphological changes in the brain tissue of the albino mice following chronic cadmium (Cd) administration.
Non-anesthetized adult male mice were given parsley juice (Petroselinum crispum, Apiaceae) daily by gastric intubation at doses of 10 and 20 g/kg/day. The animals were divided into six groups: Group A, mice were exposed to saline; Groups B and C, were given low and high doses of parsley juice, respectively; Group D, mice were exposed to Cd; Groups E and F, were exposed to Cd and concomitantly given low and high doses of parsley, respectively.
Cd intoxication can cause behavioral abnormalities, biochemical and histopathological disturbances in treated mice. Parsley juice has significantly improved the Cd-associated behavioral changes, reduced the elevation of lipid peroxidation and normalized the Cd effect on reduced glutathione and peroxidase activities in the brain of treated mice. Histological data have supported these foundations whereas Cd treatment has induced neuronal degeneration, chromatolysis and pyknosis in the cerebrum, cerebellum and medulla oblongata.
The low dose (5 g/kg/day) of parsley exhibited beneficial effects in reducing the deleterious changes associated with Cd treatment on the behavior, neurotransmitters level, oxidative stress and brain neurons of the Cd-treated mice.
Cadmium (Cd) is among the most hazardous heavy metals that is not chemically degraded in the environment and can enter into the food chain . Both natural and anthropogenic sources of this heavy metal, including industrial emissions and the application of fertilizer and sewage sludge to farm land, may lead to the contamination of soils and the increased Cd uptake by crops and vegetables grown for human consumption . Some important sources of Cd exposure for humans can be the emissions from industries of petroleum mining, batteries, metal plating, pigments, plastics, toys and alloy, cigarette smoking and through dietary consumption . Total human intake of Cd from food has been estimated by Järup  as 2.8–4.2 µg/kg body weight/week, which equates to approximately 40–60 % of the current provisional tolerable weekly intake of 7 µg/kg body weight/week.
Cd has no essential biochemical roles, but can exert diverse and severe toxicity in multiple body systems as it can bind to tissues, trigger oxidative stress, affect endocrine function, block aquaporins, and interfere with functions of essential cations such as magnesium and zinc . Cd can pose some particular risks to the young, as exposures in early life can compromise development, with lifelong physical, intellectual, and behavioral impairments . The International Agency for Research on Cancer classified cadmium as a well-known carcinogen . Cd represented one of the most toxic and carcinogenic heavy metals . It is considered as a serious health hazard to humans and other animals . Exposure to Cd may cause lesions in many organs such as brain, liver, kidney and testis , thus leading to cerebral, hepatic, renal and testicular dysfunction . It has been reported that Cd exposure produced long-term impairments of neurobehavioral status such as alterations in attention and memory as well as in the psychomotor and visuomotor functioning and speed in workers [12, 13]. Clinical data have shown aggressive elevation and anxiety-like behaviors, impaired learning and memory processes, and changes in the development of the visual system . Previous studies on Cd toxicity have reported behavioral impairments in both animal models and humans after exposure to Cd . Acetylcholine esterase is an important enzyme that hydrolyses the neurotransmitter acetylcholine in the synaptic cleft of cholinergic synapses and neuromuscular junctions . Alterations in the acetylcholine activity in various diseases and poisonings suggested that this enzyme could be an important physiological and pathological biomarker [17, 18].
Additionally, Cd can increase blood–brain barrier permeability, thus penetrating and accumulating in brain tissue of animals  and leading to brain intracellular accumulation, cellular dysfunction, and cerebral edema. Also, it can affect the degree and balance of excitation–inhibition in synaptic neurotransmission as well as the antioxidant levels in animal’s brain [19, 20]. Cd toxicity is partly due to oxidative DNA damage associated with the increased production of reactive oxygen species (ROS), such as superoxide ion, hydroxyl radicals and hydrogen peroxide . Previous studies have indicated that Cd can decrease antioxidant enzyme levels . Free radicals cause the oxidation of biomolecules (e.g., protein, amino acids, lipid and DNA), which leads to cell injury and death . For example, ROS markedly alter the physical, chemical, and immunological properties of superoxide dismutase (SOD), which further exacerbates oxidative damage in cells. This has raised the possibility that antioxidants could acts as prophylactic agents against many pathological conditions. It has long been recognized that naturally occurring substances in higher plants have antioxidant activities. Many culinary herbs (e.g., parsley) have been shown to function as natural antioxidants .
Parsley (Petroselinum crispum, Apiaceae) is an annual herb, which is important dietary source of vitamins and essential metals. It’s usage at sufficient levels whether cooked or not cooked can promote the levels of vitamins and essential metals in human body, which in turn can decrease the risks of Cd toxicity . Phytochemical screening of parsley has revealed the presence of some compounds such as flavonoids , carotenoids , ascorbic acid  and tocopherol . These components of fresh parsley leaf can scavenge superoxide anion in vitro and hydroxyl radical in addition to protecting against ascorbic acid-induced membrane oxidation . Supplementation of diets with fresh parsley leaf can increase antioxidant capacity of rat plasma and decrease oxidative stress in humans . Similarly, aqueous and ethanol extracts of fresh parsley leaf strongly inhibit linoleic acid oxidation and lipid oxidation . Biological mobility, tissue concentrations, and excretion of Cd are determined by oxidation state, solubility, a complex set of equilibria between complexing sites, as well as active transport through membranes . Chelation is central to natural detoxification of heavy metals, via formation of complexes, particularly with glutathione and other small molecules and their excretion . Essential oil extracted from parsley possessed a certain degree of antioxidant activities in terms of β-carotene bleaching capacity and free radical scavenging activity . Therefore, parsley was also reported as a possible source of antioxidants which may prevent Cd toxicity.
Also, parsley is one of the most used medicinal plants to treat arterial hypertension ; diabetes, cardiac  and renal diseases . Moreover, in experimental studies, it has been reported that this herb has strong diuretic , anti-hyperglycemic, anti-hyperlipidemic, anticoagulant , anti-oxidant , anti-microbial  and laxative activities . It has been reported that parsley alcoholic extract has a protective effect against toxicity induced by sodium valproate in male rats . Parsley leaves were used for treatment of constipation, jaundice, colic, flatulence edema, rheumatism. It was used to treat eczema, knee, ache, impotence and bleeding . However, according to our knowledge, the reported literatures on the protective effect of parsley against Cd neurotoxicity are still limited.
In the present work, we investigated the hypothesis that parsley juice may protect against Cd-induced pathological changes in albino mice.
Cadmium chloride (CdCl2) was of analytical grade and purchased from Sigma Chemical Company (St Louis, MO, USA).
Parsley juice preparation
petroselinum crispum (mill.) nymex a.w. hill from the family Apiaceae (alt. Umbelliferae) is commonly known as parsley. The origin of parsley is from Mediterranean region, but today is cultivated wherever of the world. Botanic identification was performed by taxonomist in the Department of botany and microbiology, Faculty of Sciences, King Saud University (Riyadh, Saudi Arabia) where a voucher specimen has been deposited (collection number AL 1021). The plain leaf parsley type daily collected from vegetable market in Riyadh (Saudi Arabia) was carefully washed under tap water. The fresh parsley juice was prepared daily using a vegetable juicer. Two concentrations of the juice were prepared: the first is 10 % juice, i.e., 10 g parsley squash in 100 ml drinking water. The second is 5 % juice, i.e., 5 g parsley squash in 100 ml drinking water. The prepared juice has been filtered using a filter paper after preparation and before drinking by the animals to remove fibers and other insoluble components.
All the experimental protocols and investigations were approved and complied with the Guide for Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85–23, revised 1996) and was approved by the Ethics Committee for Animal Experimentation at King Saud University (Permit Number: PT 983).
Animals and dosing schedule
A total of 48 adult male albino mice (Mus musculus) weighing 30–35 g were obtained from College of Pharmacy, King Saud University, Saudi Arabia and housed in stainless steel wire cages (5 animals/cage) under specific pathogen-free conditions. The animals were maintained at 22–25 °C on a 12:12 h light/dark cycle and provided with food and water ad libitum. Parsley juice was orally administered daily to non-anesthetized parsley treated groups by gastric intubation at two doses of 10 and 20 g/kg/day for 28 days (D). Totally, 30 mg/kg of CdCl2 dissolved in saline was intraperitoneally injected to partially anesthetized Cd treated groups by three exposure times D1, D7 and D15 (10 mg/kg every time). The animals were labeled into six groups as follows:
Group A: mice were given with tab water orally and saline intraperitoneally (Control group).
Group B: mice were given with 5 % Parsley juice orally and saline intraperitoneally (5 % Parsley group).
Group C: mice were given with 10 % Parsley juice orally and saline intraperitoneally (10 % Parsley group).
Group D: mice were given with tab water orally and Cd doses intraperitoneally (Cd intoxicated group).
Group E: mice were given with 5 % Parsley juice orally and Cd doses intraperitoneally (5 % Parsley-Cd group).
Group F: mice were given with 10 % Parsley juice orally and Cd doses intraperitoneally (10 % Parsley-Cd group).
Cd estimation assay
The analytical determination of Cd in mice brain was carried out by ICP-MS (inductively coupled plasma mass spectrometer): ELAN 9000 (Perkin Elmer Sciex Instrumento, Concord, Ontario, Canada).
Nitric acid (69 % v/v), super purity grade was supplied from Romil, England. Hydrochloric acid (37 % v/v) and hydrofluoric acid (40 % v/v) were supplied from Merck (Germany). High purity water obtained from Millipore Milli-Q water purification system was used throughout the work.
The ICP-MS calibration was carried out by external calibration with the blank solution and three working standard solutions (20, 40 and 60 ppm), starting from 1000 mg/l single standard solutions for ICP-MS (A ristar grade, BDH laboratory supplies, England for Cd).
Sample collection and preparation
Samples were prepared by accurately weighing 200 mg of longitudinal section of brain into a dry and clean Teflon digestion beaker, 6 ml of HNO3, 2 ml HCl and 2 ml HF were added to the Teflon beaker. Samples were digested on the hot plate at 120–150 °C for approximately 40 min. The resulting digest was not clear, so it was filtered through whatman filtered paper No. 42. The filtered digest was transferred to a 50 ml plastic volumetric flask and made up to mark using deionized water. A blank digest was carried out in the same way.
8 male mice from each group were used in the present study at the beginning of the 5th week (D28–30) after finishing the dosing period. The animals were used only in one test of the present tests per day. For the tests, the animals were brought in a room (25 °C) of dim red light reserved for that purpose. All tests were conducted blindly by the same experimenter .
T-maze conducting assay
The six groups of animals were prevented from food all the night before this examination. The elevated T-maze consisted of three closed arms to be T like structure. The main arm (100 × 10 × 20 cm) and two lateral arms (40 × 10 × 20 cm) at an elevation of 20 cm above the floor. Arms of the maze form T like structure. The rodent food was placed at the end of the right lateral arm. The maze was cleaned with a 20 % ethanol after each test. The hungry animals were placed in the terminal end of the main arm of the elevated T-maze facing the passage to the two lateral arms. The mice left to explore the maze for 1 min, then the animal removed from the maze and kept in its cage for 2 h. The mice replaced in the same position in the main arm and the behavior analyzed for 5 min by an experimenter who is blind for the experimental protocol. The frequency and duration in food and main arm visits (under red illumination) were recorded. The time spent exploring the arms to reach food (seconds), and the time spent in food arm in seconds, were determined. The frequency and time of entering the food lateral arm not the other lateral arm was considered to be memory reflector according to Leret .
Cage activity assay
The Ugo Basile 47420-Activity Cage was used to record spontaneous co-ordinate activity of mice and variation of this activity in time either horizontal or vertical movements. This test was performed for 3 min/animal.
Grip-strength meter assay
The Ugo Basile 47200-Grip-Strength Meter suitable for mice automatically measures grip-strength (i.e. peak force and time resistance) of forelimbs in mice. The aim was to assess forelimbs muscle strength. Each animal was tested for three times and the peak force of each mouse was recorded. The mean of three values of each mouse was recorded.
The Ugo Basile rota-rod instrument was used in this test, the mouse is placed on a horizontally oriented and mechanically rotating at 15 rpm rod. The rod is suspended above a cage floor, which is low enough not to injure the animal, but high enough to induce avoidance of fall. Mice naturally try to stay on the rotating rod, or rota-rods, and avoid falling to the ground. The length of time that a given animal stays on this rotating rod is a measurement of their balance, coordination, physical condition, and motor-activity.
8 animals of each group were anesthetized by light ether and sacrificed at D30. Brain was dissected and 0.5 g tissue was homogenized in 5 ml of cold 0.1 M HClO4 containing 0.05 % EDTA. The homogenate was centrifuged at 10,000 rpm for 10 min at 4 °C and the clear supernatant collected in a microfuge tube (0.5 ml each) and stored at −40 °C until assays.
Dopamine and serotonin determination
The level of neurotransmitters 5-hydroxytryptamine or serotonin and dopamine was estimated in the brain. The monoamines dopamine and serotonin was estimated using the modified method of Patrick . A 10 % homogenate of the brain has been re-centrifuged at 17,000 rpm at 4 °C for 5 min. The supernatants were filtered using 0.45 μm pore filters and analyzed by high performance liquid chromatography. The mobile phase consisted of 32 mM citric acid monohydrate, 12.5 mM disodium hydrogen orthophosphate, 7 % methanol, 1 mM octane sulfonic acid and 0.05 mM EDTA. The mobile phase was filtered through 0.22 μm filter and degassed under vacuum before use. Bondpak C18 column was used at a flow rate of 1.2 ml/min and the injection volume of the sample was 20 μl. The levels of dopamine and serotonin were calculated using a calibration curve and the results were expressed as ng/mg tissue weight.
Determination of acetylcholine
The method has been described previously which didn’t use acetylcholine esterase inhibitor . Inbrief, dialysate samples were directly injected into the liquid chromatography/electrochemistry system assisted by a chromatography manager (Millennium; Waters, Milford, MA) and analyzed for acetylcholine. Acetylcholine was separated on a coiled cation exchanger acetylcholine column (analytical column) (Sepstik 530 × 1.0 mm I.D., packed with polymetric strong exchanger, 10 μm in diameter; BAS, West Lafayette, IN), followed by the post-immobilized enzyme reactor which consisted of choline oxidase/acetylcholine esterase. Acetylcholine was hydrolyzed by acetylcholine esterase to form acetate and choline in the post-immobilized enzyme reactor, and then choline was oxidized by choline oxidase to produce betaine and hydrogen peroxide (H2O2). H2O2 is detected via oxidation of horseradish peroxidase, which in turn oxidized Os (bpy) entrapped in the redox polymer coated on the surface of the glassy carbon electrode (MF-9080; BAS), set at +100 mV (LC-4C; BAS) versus Ag/AgCl reference electrode. This reduction was analyzed with the detector (LC-4C; BAS) as a signal indicating acetylcholine on the chromatogram.
Lipid peroxidation assay (TBARS)
Lipid peroxidation was determined by assaying thiobarbituric acid-reactive substances (TBARS) according to the method of Preuss . Briefly, 1.0 ml supernatant was precipitated with 2 ml 7.5 % trichloroacetic acid and centrifuged at 1000g for 10 min. Clear supernatant was mixed with 1 ml 0.70 % thiobarbituric acid, incubated at 80 °C and the absorbance measured at 532 nm. Tetramethoxypropane was used as the standard.
Glutathione (GSH) assay
Glutathione content was determined according to the procedure of Beutler  with some modifications. Briefly, 0.20 ml of tissue supernatant was mixed with 1.5 ml precipitating solution containing 1.67 % glacial metaphosphoric acid, 0.20 % Na-EDTA and 30 % NaCl. The mixture was allowed to stand for 5 min at room temperature and centrifuged at 1000g for 5 min. One ml clear supernatant was mixed with 4 ml 0.30 M Na2HPO4 and 0.50 ml DTNB reagent (40 mg 5, 5′ dithiobis-(2-nitrobenzoic acid dissolved in 1 % sodium citrate). A blank was similarly prepared in which 0.20 ml water was used instead of the brain supernatant. The absorbance of the color was measured at 412 nm in a spectrophotometer.
Peroxidase activity determination
Peroxidase activity was determined according to the method of Kar and Mishra . Briefly, 1.0 ml supernatant was mixed with 3.0 ml of 0.01 M phosphate- buffered saline (pH 6.8), 315 μl of 2 % pyrogallol, 154 μl H2O2 and incubated for 15 min at 25 °C. The reaction was stopped by the addition of 0.50 ml of 5 % H2SO4 and the absorbance was recorded at 420 nm. Peroxidase activity was expressed as the amount of purpurogallin formed per unit absorbance.
For the histological preparations, left loop of cerebellum, cerebral cortex and medulla oblongata of three sacrificed animals were immediately fixed in 20 % formalin saline for 24 h. The tissues were washed to remove the excessive fixative and then dehydrated in ascending grades (70, 80, 90 and 95 %) of ethyl alcohol for 45 min each, then in two changes of absolute ethyl alcohol for 30 min each. This was followed by two changes of xylene for 30 min each. The tissues were then impregnated with paraplast plus (three changes) at 60 °C for 3 h and then embedded in paraplast plus. Sections (4–5 µm) were prepared with a microtome, de-waxed, hydrated and stained in Mayer’s haemalum solution for 3 min. The sections were stained in Eosin for one min, washed in tap water and dehydrated in ethanol as described above. Hematoxylin and eosin (H&E) stained sections were prepared according to the method of Mallory .
The Statistical Package for the Social Sciences (SPSS for windows version 11.0; SPSS Inc, Chicago) was used for the statistical analyses. Comparative analyses were conducted by using the general linear models procedure (SPSS, Inc). Also, the data were analyzed using one-way and two-way analysis of variance (ANOVA) followed by LSD computations to compare various groups with each other. Results were expressed as mean ± S.D. The level of significance was expressed as significant at P < 0.05, highly significant at P < 0.01 and very highly significant at P < 0.001.
Cd concentrations in the brain tissues of exposed mice were estimated. As shown in Fig. 1, Cd bioaccumulation in the brain of both parsley groups (Groups B and C) was significantly (P < 0.05) reduced in comparison to the control group. On the other hand, a highly significant (P < 0.001) increase in the level of Cd in Cd-intoxicated group (Group D) in comparison to the control group, was monitored. Meanwhile, parsley treatment has an obvious ameliorating effect on Cd-intoxication. Both the low (5 %) and the high (10 %) doses of parsley have clearly decreased the Cd level in the brain tissues of Cd-parsley groups in comparison to the Cd-group (Group D) with the more reducing effect being associated with the low parsley dose (Group E).
Effect of parsley treatment on the body weight change in Cd- treated mice
Toxicological studies have illustrated that many toxicants are usually associated with weight loss in exposed animals. In the current study, we have investigated the dampening effect of Cd toxicity on the body weight increase in treated mice and the possible restoring capacity of parsley on this effect. Table 1 illustrates that both parsley groups (B and C) have exhibited a similar pattern of body weight change in comparison to the control group (A) during the experiment time. Conversely, Cd-intoxicated group (D) has showed a significant decrease in the body weight in comparison to the control group. Parsley treatment has restored to some extent, the normal pattern of body weight change seen in the control groups.
The behavior of animals in the T-maze showed that the Cd treated animals have bad memory and low smell ability. This was elucidated by the decreased number of entrances to the main arm (Fig. 2a) and to the food arm (Fig. 2c) concomitant with the long time consumed by the Cd-treated animals to reach to the food (Fig. 2b) and to the food arm (Fig. 2d) in comparison to the control animals. Parsley treatment has showed an ameliorating effect that was clearer in the low dose parsley group in comparison to the high dose one. In the activity cage, the animals of Cd-intoxicated group showed a significant elevation in the vertical and horizontal movements as compared to the control group. On the contrary of the vertical movement, the animals of both parsley groups (Groups B and C) showed significant increases in the values of horizontal movements. After parsley treatment, the animals of Groups E and F displayed values of vertical and horizontal movements that were near to that of the control group animals (Fig. 3a, b).
The fore-limb muscles of mice in Groups A, B and C recorded relatively similar beaks in the grip strength examination scores. The recorded beaks of Group D animals appeared significantly lower in comparison to the control, the beaks achieved by the animals of Groups E and F showed significant improvements (Fig. 3c).
In rota-rod test and in comparison to the control group, the latency to fall by the Cd-intoxicated group on the rod was significantly reduced. Parsley treatment has resulted in increasing the staying times of the animals of Groups E and F on the rod to become near to that of the control group (Fig. 3d).
Dopamine is an important neurotransmitter and its concentration is usually linked with the functioning of the nervous system and the behavior of living organisms. In both of the control and the parsley groups (A, B and C), the dopamine level in mice brain appeared nearly similar with little elevation in Group C. In comparison to the control group, a highly significant depletion of dopamine concentration has been detected in Cd-intoxicated group (P < 0.001). Parsley treatment has slightly ameliorated this effect but still, a significant reduction in Groups E and F (P < 0.01)was recorded (Fig. 4a). Serotonin level, another important neurotransmitter, was investigated in all the experimental groups. Both parsley groups (B and C) have showed no significant difference in the brain level of serotonin in comparison to the control group. A significant reduction in the level of serotonin was detected in Cd-intoxicated group (D). Following parsley treatment with two different doses, the level of serotonin was significantly reduced in the low dose parsley group (E) while the high dose one (F) has showed no significant reduction as compared to the control (Fig. 4b).
The present acetylcholine concentrations showed similar results to dopamine. In Groups A, B and C, its concentration did not show any significant difference, while it displayed a highly significant (P < 0.01) depletion in Cd-intoxicated group. In Groups E and F, a significant (P < 0.05) reduction in serotonin level was also monitored but still, parsley treatment has an obvious lowering effect on the Cd-induced pathology (Fig. 4c).
As illustrated in Fig. 5a, the change in brain lipid peroxidation in parsley groups was not significant in comparison to the control group. Conversely, a significant (P < 0.01) elevation in TBARS level was detected in Cd-intoxicated group. However, after parsley treatment the TBARS level was significantly lowered in both Cd-parsley groups (Groups E and F) in comparison to Cd group (Group D).
In addition to the increased level of TBARS, Cd treatment has also produced a significant (P < 0.001) decrease in GSH content in Cd-intoxicated group. Parsley has exerted a positive impact and improvement was observed in both of parsley-Cd groups as indicated from the significant increase in Groups E and F in comparison to the Cd group (D) (Fig. 5b).
Peroxidase activity was also monitored as an important indicator for oxidative stress. While both parsley groups have a similar level of peroxidase in comparison to the control, Cd-intoxicated group has showed a significant elevation in the peroxidase activity as compared to the control (Fig. 5c). After parsley treatment, both parsley-Cd groups have showed decreased level of peroxidase as compared to the Cd group.
Brain histopathological changes
The normal pyramidal neurons exhibited their general characteristic shape. The nuclei of these cells were rounded, large and centrally located (Fig. 6). The normal cells of the cerebral cortex had spherical or pyramidal perikaryon, whose nuclei were large; also the neurons were arranged in a regular pattern (Fig. 6a–c). The cerebral neurons appeared more developed toward the white matter. In Cd-treated group, chromatolysis and pyknosis have been observed in the pyramidal neurons. In Groups E and F, parsley juice showed significant neuronal protection through reducing the rate of chromatolysis and pyknosis (Fig. 6d–f).
In the cerebellum, the fold layers (molecular, Purkinje cells and internal granular) became completely mature and the external granular layer disappeared completely (Fig. 7). The neuronal density in the molecular layer of both the control and the parsley treated groups was the highest as compared to Cd-treated groups. The normal Purkinje cells were arranged in a single row of large neurons with pear-shaped perikaryon and large nucleus. The lateral processes were disappeared and the apical processes formed the permanent dendritic tree (Fig. 7). In Cd-treated groups, some degenerated and pyknotic Purkinje cells were detected. Of which, some were more spindle-shaped and small. These numbers of degenerated Purkinje neurons were reduced in Cd-parsley groups (Groups E and F) (Fig. 7d–f). Variations have been observed in the folds size of the groups, where small folds appeared in Group D.
The normal medulla neurons appeared large in size, polygonal, varied in shape and had round nuclei (Fig. 8a–c). In Cd-treated group, most medulla neurons appeared small and pyknotic (Fig. 8d). In Cd-parsley treated groups, the medulla neurons showed improvement (Fig. 8e, f).
The present study was designed to investigate the protective role of parsley juice against Cd neurotoxicity in albino mice. The obtained results suggested that intake of parsley may partly improve the malformations induced by exposure of adult mice to Cd. The effects of daily supplementation of two different doses of parsley on the deleterious changes of Cd on animal’s behavioral activities, neurotransmitters level, oxidative stress parameters and histopathology of brain were investigated in the current study. The high concentration of Cd in brain tissues of Cd-treated groups may be due to the Cd bioaccumulation. The current results of Groups E and F showed that parsley juice has an observable protective effect against Cd accumulation especially at the low dose. This may be due to the significant effect of parsley in the excretion of heavy metals from body, an effect that was documented before . In fact the dose dependence of parsley has been illustrated before  and it seems in the current study that the low parsley dose is considered as a therapeutic dose while the high dose one is considered as overdose. Previously, animal’s body weight was known to be one of the most sensitive indicators of toxicity . In a recent study of Gonçalves , the rodents exposed to Cd were reported to display a lowered body weight. Our data supports these previous foundations in Cd-intoxicated group. However, in Cd-parsley groups, parsley could improve this effect and this may be attributed, in part, to the beneficial actions that parsley exerts on the gastrointestinal tract .
The results of the present study showed that the brain levels of neurotransmitters were significantly depleted by Cd treatment in Cd-exposed mice. It was reported previously that neurotransmitter depletion is nutritionally based. Neurotransmitters are made from amino acids that are required for its creation. Consequently, if the diet is deficient, neurotransmitter deficiency develops . Here, Cd-intoxication may have been resulted in anorexia and consequently diet deficiency. There is an evidence of an inhibitory role of dopamine and serotonin mediated receptors in depressing the hyperexcitability of brain neurons as appeared by the poor performance of the treated animals in the current behavioral examinations . Recently, many research groups have focused on the participation of serotonin in the neurochemical mechanisms of cognition, especially of learning and memory. Potential toxic mechanisms of action for Cd may include the disruption in serotonergic neurotransmission through disturbed levels of neurotransmitters in mice brain . It was reported before that Cd toxicity has resulted in disrupted acetylcholine esterase activity . Cd-intoxicated group of animals showed greater oxidative stress and a marked depletion of the antioxidants, than Groups E and F animals that were exposed to Cd and parsley juice. The results presented in the current study showing higher increase of the lipid peroxidation in Group D which is in accordance with the results obtained by Méndez-Armenta and Ríos  that reported an enhanced lipid peroxidation and increased TBARS after acute Cd exposure. Glutathione is one of the essential compounds for maintenance of cell integrity and participation in cellular metabolism . Alterations in the ratio of reduced (GSH) and oxidized (GSSG) glutathione are well accepted as one of the indicators of oxidative stress in humans and experimental animals [52, 53].
The depletion of GSH may lead to lipid peroxidation. Therefore, GSH is considered as an important biomarker of oxidative stress . The level of GSH is regulated by NADPH dependent enzyme, glutathione reductase (GR), and therefore an inhibition of GR may adversely affect GSH levels . Furthermore, conjugation of heavy metals metabolites, with GSH forms glutathione-S-conjugates, which ultimately forms mercapturic acids . This may further deplete GSH in the cell. Abu-Taweel  reported that Cd depleted GSH content and increased peroxidase enzymes activities in mice. Increased peroxidase activity might be due to generation of free radicals . The antioxidant enzymes (e.g. peroxidases) constituted a mutually supportive team of defense against ROS .
Several mechanisms can contribute to the increased oxidative stress in toxicity induced by heavy metals, especially chronic exposure to Cd. Accumulated evidence pointed out that Cd inhalation can lead to elevated ROS and reactive nitrogen species (RNS) production by the mitochondrial respiratory system, antioxidant enzyme inactivation and an imbalance of glutathione redox status . Cd toxicity can promote an important oxidative imbalance, favoring the production of free radicals and the reduction of antioxidant defenses. At high concentrations, ROS/RNS can damage the major components of the cellular structure, including nucleic acids, proteins, amino acids, and lipids . Such oxidative modifications would affect several cell functions, metabolism, and gene expression, which in turn can cause some other pathological conditions . The oxidative stress leads to neuronal damage in several brain regions [54–59]. For example, neuronal loss in the cerebrum can impair animal’s memory  while, neuronal loss in the cerebellum can have some adverse effects on balance and coordination . In addition, neuronal loss in the medulla oblongata and the spinal cord can affect physical activity of mice . Supplementation with parsley juice for 28 days alleviated somewhat the Cd-induced toxicity, which showed significant improvement in the physical balance, memory, coordination, motor activities, muscles strength and brain neurotransmitters levels in Cd-treated animals. Parsley supplementation also restored GSH balance and decreased lipid peroxidation and peroxidase activity. Overall, this study demonstrated that the low dose (10 g/kg/day) of parsley supplementation could improve the pathological alterations in mice and this is in accordance with the results reported by Zhang . Parsley has also been found to significantly suppress hydroperoxide and ROS levels in brain and other tissues in mice by stimulating production of glutathione synthesis and thereby boosting cellular antioxidant defenses . Taken together, our data demonstrates that parsley may be an important therapeutic tool to combat Cd toxicity-associated effects. This parsley ameliorating effect may be due to its ability to neutralize free radicals and thereby prevent neuronal damage caused by oxidative stress.
Parsley has protective effects against Cd neurotoxicity in albino mice. Parsley juice supplementation improves the abnormal behavior of Cd intoxicated mice and reduces neuronal aberrations in the brain.
ethylene diamine tetraacetic Acid
inductively coupled plasma mass spectrometer
reactive oxygen species
reactive nitrogen species
thiobarbituric acid-reactive substances
ATSDR. Agency for toxic substance and disease registry. Atlanta: U.S. Toxicological Profile for Cadmium, Department of Health and Humans Services, Public Health Service, Centers for Disease Control; 2005.
Järup L, Akesson A. Current status of cadmium as an environmental health problem. Toxicol Appl Pharmacol. 2009;238:201–8.
de Souza PF, Diamante MA, Dolder H. Testis response to low doses of cadmium in Wistar rats. Int J Exp Pathol. 2010;91(2):125–31.
Järup L, Berglund M, Elinder CG, Nordberg G, Vahter M. Health effects of cadmium exposure—a review of the literature and a risk estimate. Scan J Work Environ Health. 1998;42:1–52.
Fowler BA. Monitoring of human populations for early markers of cadmium toxicity: a review. Toxicol Appl Pharmacol. 2009;238(3):294–300.
Sears ME. Chelation: harnessing and enhancing heavy metal detoxification—a review. ScientificWorldJournal. 2013;2013:1–13.
Matovic VA, Bulat ZB, Cosi D. Cadmium toxicity revisited: focus on oxidative stress induction and interactions with zinc and magnesium. Arch Ind Hyg Toxicol. 2011;62:65–76.
Abu-Taweel GM, Ajarem JS, Ahmad M. Protective effect of curcumin on anxiety, learning behavior, neuromuscular activities, brain neurotransmitters and oxidative stress enzymes in cadmium intoxicated mice. J Behav Brain Sci. 2013;3:74–84.
Brzoska MM, Majewska K, Kupraszewicz E. Effects of low, moderate and relatively high chronic exposure to cadmium on long bones susceptibility to fractures in male rats. Environ Toxicol Pharmacol. 2010;29(3):235–45.
Xu LC, Sun H, Wang SY, Song L, Chang HC, Wang XR. The roles of metallothionein on cadmium induced testes damages in Sprague dawley rats. 2005.
Ognjanovic BI, Markovic SD, Ethordevic NZ, Trbojevic IS, Stajn AS, Saicic ZS. Cadmium induced lipid peroxidation and changes in antioxidant defense system in the rat testes: protective role of coenzyme Q(10) and vitamin E. Reprod Toxicol. 2010;29(2):191–7.
Viaene MK, Masschelein R, Leeders J, De Groof M, Swerts LJVC, Roels HA. Neurobehavioural effects of occupational exposure to cadmium: a cross sectional epidemiological study. Occup Environ Med. 2000;57:19–27.
Haider S, Anis L, Batool Z, Sajid I, Naqvi F, Khaliq S, Ahmed S. Short term cadmium administration dose dependently elicits immediate biochemical, neurochemical and neurobehavioral dysfunction in male rats. Metab Brain Dis. 2015;30(1):83–92.
Terçariol SG, Almeida AA, Godinho AF. Cadmium and exposure to stress increase aggressive behavior. Environ Toxicol Pharmacol. 2011;32:40–5.
Gonçalves JF, Fiorenza AM, Spanevello RM, Mazzanti CM, Bochi GV, Antes FG, Stefanello N, Rubin MA, Dressler VL, Morsch VM, Schetinger MRC. N-Acetylcysteine prevents memory deficits, the decrease in acetylcholinesterase activity and oxidative stress in rats exposed to cadmium. Chem Biol Interact. 2010;186:53–60.
Soreq H, Seidman S. Acetylcholinesterase—new roles for an old actor. Nat Rev Neurosci. 2001;2:294–302.
Gonçalves JF, Fernando T, da Costa NP, Farias GJ, et al. Behavior and brain enzymatic changes after long-term intoxication with cadmium salt or contaminated potatoes. Food Chem Toxicol. 2012;50:3709–18.
Schmatz R, Mazzanti CM, Spanevello R, Stefanello N, Gutierres J, Corrêa M, Rosa MM, Rubin MA, Schetinger MRC, Morsch VM. Resveratrol prevents memory deficits and the increase in acetylcholinesterase activity in streptozotocin-induced diabetic rats. Eur J Pharmacol. 2009;610:42–8.
Méndez-Armenta M, Ríos C. Cadmium neurotoxicity. Environ Toxicol Appl Pharmacol. 2007;23:350–8.
Ji LY, Zhang WW, Yu D, Cao YR, Xu H. Effect of heavy metal-solubilizing microorganisms on zinc and cadmium extractions from heavy metal contaminated soil with Tricholomalobynsis. World J Microbiol Biotechnol. 2012;28(1):293–301.
Stohs SJ, Bagchi D, Hassoun E, Bagchi M. Oxidative mechanism in the toxicity of chromium and cadmium ions. J Environ Pathol Toxicol Oncol. 2000;19:201–13.
Paniagua-Castro N, Escalona-Cardoso G, Madrigal-Bujaidar E, Martínez-Galero E, Chamorro-Cevallos G. Protection against cadmium-induced teratogenicity in vitro by glycine. Toxicol In Vitro. 2008;22(1):75–9.
Jaswir I, Che Man YB, Kitts DD. Synergistic effect of rosemary, sage and citric acid on retention of fatty acids fromre fined, bleached and deodorized palm olein during repeated deepfat frying. J Am Oil Chem Soc. 2000;77:527–33.
Zhai Q, Narbad A, Chen W. Dietary strategies for the treatment of cadmium and lead toxicity. Nutrients. 2015;7:552–71.
Fejes S, Blazovics A, Lemberkovics E, Petri G, Szöke E, Kery A. Free radical scavenging and membrane protective effects of methanol extracts from Anthriscuscerefolium L. (Hoffm.) and Petroselinum crispum (Mill.) nym. ex A.W. Hill. Phytother Res. 2000;14:362–5.
Francis GW, Isaksen M. Droplet counter current chromatography of the carotenoids of parsley: Petroselinum crispum. Chromatographia. 1989;27:549–51.
Davey MW, Bauw G, Montagu MV. Analysis of ascorbate in plant tissues by high-performance capillary zone electrophoresis. Anal Biochem. 1996;239:8–19.
Fiad S, El Hamidi M. Vitamin E and trace elements. SeifenOeleFetteWasche. 1993;119:25–6.
Nielsen SE, Young JF, Daneshvar B, Lauridsen ST, Knuthsen P, Sandstrom B, et al. Effect of parsley (Petroselinumcrispum) intake on urinary apigenin excretion, blood antioxidant enzymes and biomarkers for oxidative stress in human subjects. Br J Nutr. 1999;81:447–55.
Wong PY, Kitts DD. Studies on the dual antioxidant and antibacterial properties of parsley (Petroselinumcrispum) and cilantro (Coriandrumsativum) extracts. Food Chem. 2006;97:505–15.
Nordberg GF, Nogawa K, Nordberg M, Friberg LT. Foreword metals–a new old environmental problem and chapter 23: cadmium. In: Nordberg GF, Fowler BA, Nordberg M, Friberg LT, editors. Handbook on the toxicology of metals. 3rd ed. Academic Press: Academic Press; 2011. p. vii–446–451, 463–470, 600–609.
Zhang H, Chen F, Wang Xi, Yao HY. Evaluation of antioxidant activity of parsley (Petroselinumcrispum) essential oil and identification of its antioxidant constituents. Food Res Int. 2006;39:833–9.
Eddouks M, Maghrani M, Lemhadri A, Ouahidi ML, Jouad H. Ethnopharmacological survey of medicinal plants used for the treatment of diabetes mellitus, hypertension and cardiac diseases in the south-east region of Morocco (Tafilalet). J Ethnopharmacol. 2002;82:97–103.
Jouad H, Haloui M, Rhiouani H, El Hilaly J, Eddouks M. Ethnobotanical survey of medicinal plants used for the treatment of diabetes, cardiac and renal diseases in the North centre region of Morocco (Fez-Boulemane). J Ethnopharmacol. 2001;77:175–82.
Darias V, Martin-Herrera D, Abdalla S, Fuente D. Plant used in urinary pathologies in the Canary island. Pharm Biol. 2001;39:170–80.
Yazicioglu A, Tuzlaci E. Folkmedicinal plants of Trabzon (Turkey). Fitoterapia. 1996;67:307–18.
Ojala T, Remes S, Haansuu P, Vuorela H, Hiltunen R, Haahtela K, Vuorela P. Antimicrobial activity of some coumarin containing herbal plants growing in Finland. J Ethnopharmacol. 2000;73:299–305.
Kreydiyyeh SI, Usta J, Kaouk I, Al-Sadi R. The mechanism underlying the laxative properties of parsley extract. Phytomedicine. 2001;8:382–8.
Jassim AM. Protective Effect of Petroselinumcrispum (parsley)extract on histopathological changes in liver, kidney and pancreas induced by sodium valproate-in male rats. Kufa J Vet Med Sci. 2013;4(1):20–7.
Manderfeld MM, Schafer HW, Davidson PM, Zottola EA. Isolation and identification of antimicrobial furocoumarins from parsley. J Food Prot. 1997;60:72–7.
Ajarem JS, Ahmad M. Behavioral and biochemical consequences of perinatal exposure of mice to instant coffee: a correlative evaluation. Pharmacol Biochem Behav. 1991;40(4):847–52.
Leret ML, San Millán JA, Antonio MT. Perinatal exposure to lead and cadmium affects anxiety-like behavior. Toxicology. 2003;186:125–30.
Patrick OE, Hirohisa M, Masahira K, Koreaki M. Central nervous system bioaminergic responses to mechanic trauma. Surg Neurol. 1991;35(4):273–9.
Ichikawa J, Li Z, Dai J, Meltzer HY. Atypical antipsychotic drugs, quetiapine, iloperidone, and melperone, preferentially increase dopamine and acetylcholine release in rat medial prefrontal cortex: role of 5-HT1A receptor agonism. Brain Res. 2002;956(2):349–57.
Preuss HG, Jarrel ST, Scheckenbach R, Lieberman S, Anderson RA. Comparative effects of chromium, vanadium and Gymnema sylvestre on sugar-induced blood pressure elevations in SHR. J Am Coll Nutr. 1998;17(2):116–23.
Beutler E, Duron O, Kelly BM. Improved method for determination of blood glutathione. J Lab Clin Med. 1963;61:882–8.
Kar M, Mishra D. Catalase, peroxidase and polyphenoloxidase activities during rice leaf senescence. Plant Physiol. 1976;57:315–9.
Mallory FB. Pathological techénique. Philadelphia: Saunders; 1988.
Yousofi A, Daneshmandi S, Soleimani N, Bagheri K, Karimi MH. Immunomodulatory effect of parsley (Petroselinumcrispum) essential oil on immune cells: mitogen-activated splenocytes and peritoneal macrophages. Immunopharmacol Immunotoxicol. 2012;34(2):303–8.
Wise LD, Gordon LR, Soper KA, Duchai DM, Morrissey RE. Developmental neurotoxicity evaluation of acrylamide in Sprague-Dawley rats. Neurotoxicol Teratol. 1995;17:189–98.
Moazedi AA, Mirzaie DN, Seyyednejad SM, Zadkarami MR, Amirzargar A. Spasmolytic effect of Petroselinumcrispum (Parsley) on rat’s ileum at different calcium chloride concentrations. Pak J Biol Sci. 2007;10(22):4036–42.
Rao TS, Asha MR, Ramesh BN, Rao KS. Understanding nutrition, depression and mental illnesses. Indian J Psychiatry. 2008;50(2):77–82.
Richter-Levin G, Segal M. The effects of serotonin depletion and raphe grafts on hippocampal electrophysiology and behavior. J Neurosci. 1991;11(6):1585–96.
Conklin KA. Dietary antioxidants during cancer chemotherapy: impact on chemotherapeutic effectiveness and development of side effects. Nutr Cancer. 2000;37(1):1–18.
Gohil K, Viguie C, Stanley WC, Brooks GA, Packer L. Blood glutathione oxidation during human exercise. J Appl Physiol. 1988;64(1):115–9.
Ajarem J, Allam AA, Ebaid H, Maodaa SN, Al-Sobeai SM, Rady AM, Metwalli A, Altoom NG, Ibrahim KE, Sabri MI. Neurochemical, structural and neurobehavioral evidence of neuronal protection by whey proteins in diabetic albino mice. Behav Brain Funct. 2015;11:7.
Allam AA, El-Ghareeb AA, Abdul-Hamid M, Gad MA, Sabri I. Effect Of prenatal and perinatal acrylamide on the biochemical and morphological changes in liver of developing albino rat. Arch Toxicol. 2010;84(2):129–41.
Allam AA, El-Ghareeb AA, Abdul-Hamid M, Bkry A, Sabri I. Prenatal and perinatal acrylamide disrupts the development of cerebellum in rat: biochemical and morphological studies. Toxicol Ind Health. 2011;27(4):291–306.
Allam AA, Ajarem J, Abdul-Hamid M, Bakry A. Acrylamide disrupts the development of medulla oblongata in albino rat: biochemical and morphological studies. Afr J Pharm Pharmacol. 2013;7(20):1320–31.
Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39(1):44–84.
Young IS, Woodside JV. Antioxidants in health and disease. J Clin Pathol. 2001;54(3):176–86.
AA and SM carried out the experiments, participated in the design of the study, performed the statistical analysis and drafted the manuscript. JA and ZW provided expertise in the HPLC–MS analysis and participated in the design and coordination of the study. MA revised the whole Ms, performed statistical revision and performed the body weight change follow up and data analysis. GA revised the discipline of the statistical work and whole manuscript. All authors read and approved the final manuscript .
The authors would like to extend their sincere appreciation to the Deanship of Scientific Research at King Saud University for its funding of this research through the Research Group Project no RGP- VPP- 240.
The authors declare that they have no competing interests.